A New Look at Mechanisms In Bioenergetics - 1st Edition - ISBN: 9780125746700, 9780323160117

A New Look at Mechanisms In Bioenergetics

1st Edition

Authors: Efraim Racker
eBook ISBN: 9780323160117
Imprint: Academic Press
Published Date: 1st January 1976
Page Count: 212
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Description

A New Look at Mechanisms in Bioenergetics features eight lectures based on the Robbins Lectures given at Pomona College in April 1973. These lectures are based mainly on the author's own laboratory work and are intended for students of biology and biochemistry who want to devote their lives to research.
Lecture 1 presents some of the general lessons learned from research in the field of bioenergetics. It also discusses methods for measuring oxidative phosphorylation and the resolution of soluble multienzyme systems. Lecture 2 explains the biochemical approach to the problem of photophosphorylation. Lecture 3 considers the intersection of oxidative phosphorylation and membranology. Lecture 4 discusses the coupling device and its partial reactions. Lecture 5 focuses on the oxidation chain in mitochondria. Lecture 6 discusses the resolution and reconstitution of oxidative phosphorylation. Lecture 7 examines the reconstitution of ion pumps. Finally, Lecture 8 covers oxidation control in glycolysis; the high aerobic glycolysis of tumor cells; ATPases in tumor cells; and the repair of ion pumps in tumor cells.

Table of Contents


Preface

Abbreviations

Lecture 1 Troubles Are Good for You

How It All Started

What Is Oxidative Phosphorylation?

How Do We Measure Oxidative Phosphorylation?

First Approaches to the Resolution of the Membrane

Alio topic Properties of F1

Electron Microscopy

Isolation of Resolved Particles

Reconstitution of Oxidative Phosphorylation

Lecture 2 Photophosphorylation

On the Origin of Life

Electron Pathway in Photophosphorylation

Further Similarities with Mitochondria

Resolution of a Coupling Factor

Proton Movements in Chloroplasts

Asymmetric Assembly of the Chloroplast Membrane

Reversal of Photophosphorylation

Lecture 3 Functions and Structure of Membranes and the Mechanism of Phosphorylation Coupled to Electron Transport

General Comments

Function and Structure of Membranes

Mechanism of Phosphorylation Coupled to Electron Transport

Experimental Approaches

Lecture 4 The Coupling Device

Partial Reactions and Components of the Coupling Device

The ATP-Driven Proton Pump

Lecture 5 The Oxidation Chain and the Topography of the Inner Mitochondrial Membrane

Analysis of the Oxidation Chain

Isolation of Complexes and Individual Catalysts

The Three Segments of the Oxidation Chain

The Topography of the Oxidation Chain

Lecture 6 Resolution and Reconstitution of Oxidative Phosphorylation

General Comments

Reconstitutions of Mitochondrial and Chloroplast Membrane Functions

Reconstitution of the Proton Pump of Halobacterium halobium and of Rhodopsin-Catalyzed Photophosphorylation

Lecture 7 Reconstitution and Mechanism of Action of Ion Pumps

General Comments

Reconstitution of Ion Pumps

What Can We Learn from Resolution and Reconstitution Experiments?

Lecture 8 Control of Energy Metabolism

Oxidation Control

The Pasteur Effect

The Competition Mechanism

High Aerobic Glycolysis in Tumor Cells (The Warburg Effect)

ATPases in Tumor Cells

Repair of Ion Pumps in Tumor Cells

Bibliography

Index




Details

No. of pages:
212
Language:
English
Copyright:
© Academic Press 1976
Published:
Imprint:
Academic Press
eBook ISBN:
9780323160117

About the Author

Efraim Racker

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