A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.
Table of Contents
Preface to Volume 5 Preface to Volume 4 Preface to Volumes 1, 2 and 3 List of Previous Contents Chapter 1. Selective Staining of Histones with Ammoniacal-Silver Chapter 2. Optical Rotatory Dispersion and Circular Dichroism Chapter 3. Ultrasensitive Reaction Calorimetry Chapter 4. Proteins at Sedimentation Equilibrium in Density Gradients Author Index Subject Index