Yeast Gene Analysis

Edited by

  • Ian Stansfield, University of Aberdeen, Foresterhill, U.K.

Edited by

  • Mike Stark, University of Dundee, U.K.

Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.
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Audience

Microbiologists and Yeast researchers (yeast molecular biologists, cell biologists) C.elegans/mouse/other eukaryote system researchers possibly seeking to utilise yeast as a simple model system

 

Book information

  • Published: May 2007
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-369478-2


Table of Contents

PrefaceIntroduction to functional analysis in yeastYeast genetics and strain constructionYeast transformationA guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting)Studying essential genes: generating and using promoter fusions and conditional allelesYeast hybrid approachesArray-based yeast two-hybrid screening for protein-protein interactionsReporter genes and their uses in studying yeast gene expressionTranscript analysis: a microarray approachGFP-based microscopic approaches for whole chromosome analysis in yeastsImmunological methodsMeasuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFPIdentification, characterization, and phenotypic analysis of covalently linked cell wall ProteinsYeast protein microarraysSmart genetic screensHigh-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiaeChemical genomic tools for understanding gene function and drug actionRNA gene analysisAnalysis of gene function of mitochondriaYeast prions and their analysis in vivoMetabolic control in the eukaryotic cell, a Systems Biology perspectivePhylogenetic footprintingSaccharomyces cerevisiae as a tool for human gene function discoveryBioinformatic prediction of yeast gene functionYeast genetic strain and plasmid collectionsYeast gene analysis: the future