Translation Initiation: Cell Biology, High-throughput and Chemical-based Approaches

  • Jon Lorsch

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the third of three on the topic of Translation Initiation includes articles written by leaders in the field.
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Biochemists and researchers in related life science disciplines.


Book information

  • Published: October 2007
  • ISBN: 978-0-12-373964-3

Table of Contents

Chapter 1In vivo deletion analysis of the architecture of a multi-protein complex of translation initiation factors. Klaus H. Nielsen and LeoŇ° Val√°Ň°ekChapter 2An approach to studying the localization and dynamics of eukarayotic translation factors in live yeast cellsSusan G. Campbell and Mark P AsheChapter 3In Vitro and Tissue Culture Methods for Analysis of Translation Initiation on the Endoplasmic ReticulumSamuel B. Stephens and Christopher V. NicchittiChapter 4Mammalian Stress Granules and Processing BodiesNancy Kedersha and Paul AndersonChapter 5Methods to Analyse MircroRNA-meciated control of mRNA translation Jennifer L. Clancy, Marco Nousch, David T. Humphreys, Belinda J. Westman, Traude H. Beilharz, Thomas PreissChapter 6Methods for studying signal-dependent regulation of translation factor activity and its role in disease Christopher ProudChapter 7Analysis of mRNA Translation in Cultured Hippocampal Neurons Yi-Shuian Huang and Joel D. RichterChapter 8Detecting ribosomal association with the 5' leader of mRNAs by Ribosome Density Mapping (RDM)Naama Eldad and Yoav AravaChapter 9Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarraysDaniel Melamed and Yoav AravaChapter 10Synthesis of Anti-Reverse Cap Analogs (ARCAs) and Their Applications in mRNA Translation and StabilityEwa Grudzien-Nogalska, Janusz Stepinski, Jacek Jemielity, Joanna Zuberek, Ryszard Stolarski, Robert E. Rhoads, Edward Darzynkiewicz*Chapter 11Methods for identifying compounds that specifically target translationLetizia Brandi, Attilio Fabbretti, Pohl Milon, Marcello Carotti, Cynthia L. Pon, Claudio O. GualerziChapter 12Identifying Small Molecule Inhibitors of Eukaryotic Translation InitiationRegina Cencic, Francis Robert, Jerry PelletierChapter 13Identification of the targets of drugs affecting eukaryotic translation initiation Jun Liu Chapter 14Purification of FLAG-tagged Eukaryotic Initiation Factor 2B Complexes, Sub-complexes and Fragments from Saccharomyces cerevisiae Sarah S. Mohammad-Qureshi, Raphael Haddad, Karen S. Palmer, Jonathan P. Richardson, Edith Gomez, Graham D. Pavitt