Transgenic Animal Technology

A Laboratory Handbook


  • Carl Pinkert, Associate Vice President for Research, Professor of Pathobiology, Auburn University, AL, USA

DescriptionA transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. Importance of TopicOne potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology.Why This TitleTransgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.
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Book information

  • Published: November 2002
  • ISBN: 978-0-12-557166-1


"...this edition covers an exceptionally wide range of aspects of transgenic technology...The major strength of the book is that it comprehensively covers most important aspects of transgenic technology. Carl Pinkert must be congratulated on bringing together a set of expert authors who have covered all essential aspects of transgenesis. A ‘must have’ for every lab involved with transgenic technology." -Andreas Kolb, Hannah Research Institute "The handbook provides an excellent distillation of practical aspects of the fast growing field of animal transgenesis...the second edition contains substantial new and updated information...chapters are well illustrated with useful figures supporting the text...the handbook will be a valuable resource for two audiences, one seeking a broad appreciation for transgenic animal technology, which can be gained through reading the handbook cover to cover, and the other seeking concise descriptions of focused techniques which can be found by selection of specific chapters...overall, the text is well edited...$75.00 and its approximately 600 pages are worth the cost." -TRANSGENICS (2003) "This handbook would be valuable to the investigators producing transgenic animal models. It is well organized and the handbook provides a thorough description of current animal transgenic technology across a host of animals."-ANALYTICAL BIOCHEMISTRY (2003)

Table of Contents

Section One
Introduction to transgenic animals.

Section Two
Transgenic animal production focusing on the mouse model.
Transgenic animal production using DNA microinjection.
Factors affecting transgenic animal production.
Gene targeting in embryonic stem cells I: History and methodology.
Gene targeting in embryonic stem cells II. Conditional technologies.
Retrovirus-mediated gene transfer.
Nuclear transfer technologies.

Section Three
Production of transgenic laboratory and domestic animal species.
Production of transgenic rats.
Production of transgenic rabbits.
Production of transgenic fish.
Production of transgenic poultry.
Production of transgenic swine by DNA Microinjection.
Production of transgenic ruminants by DNA Microinjection.
Production of transgenic non-human primates.
Retrovirus-mediated gene transfer into oocytes.

Section Four: Molecular biology, analyses and enabling technologies
Vector design for Transgene Expression.
Analysis of transgene integration.
PCR optimization for Detection of Transgenic Integration.
Evaluation of Transgene Expression.
Control of gene expression (from standard gain/loss of function to anti-sense to PNAs).
Enabling technologies: Cryopreservation of gametes.
Enabling technologies: Embryo culture considerations.
Databases, Internet Resources and Genetic Nomenclature.