Techniques in Confocal Microscopy

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.
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Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.


Book information

  • Published: June 2010
  • ISBN: 978-0-12-384658-7

Table of Contents


Part I. Practical Considerations and Equipment

1. Theoretical Basis of Confocal Microscopy
Robert H. Webb

2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope
Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong

3. Equipment for Mass Storage and Processing of Data
Guy Cox

4. Antifading Agents for Confocal Fluorescence Microscopy
Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh

5. Mounting Techniques for Confocal Microscopy
Manabu Kagayama and Yasuyuki Sasano

6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy
Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe
7. Use of Confocal Microscopy to Investigate Cell Structure and Function
Ghassan Bkaily, Danielle Jacques

8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy
Xuejun Sun

Part II. Functional Approaches

9. Volume Measurements in Confocal Microscopy
Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

10. Quantitation of Phagocytosis by Confocal Microscopy
George F. Babcock and Chad T Robinson MS

11. Receptor-Ligand Internalization
Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti

12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy
David W. Piston and Susan M. Knobel

13. Trafficking of the Androgen Receptor
Virginie Georget, BĂ©atrice Terouanne, Jean-Claude Nicolas, and Charles Sultan

14. Localization of Proteases and Peptide Receptors by Confocal Microscopy
Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa

15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton

16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye
Barry R. Masters and Matthias Böhnke

17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method
Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel

18. Identification of Viral Infection by Confocal Microscopy
David N. Howell and Sara E. Miller

19. Membrane Trafficking
Sabine Kupzig, San San Lee, and George Banting

III. Green Fluorescent Protein

20. Monitoring of Protein Secretion with Green Fluorescent Protein
Christoph Kaether and Hans-Hermann Gerdes

21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters
Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li

22. Measuring Protein Degradation with Green Fluorescent Protein
Stephen R. Cronin and Randolph Y. Hampton

23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions
Gordon L. Hager

24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates
Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron

25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry
Lonnie Lybarger and Robert Chervenak

26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons
L. A. C. Blair, K. K.Bence, and J. Marshall

27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes
Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba

28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells
John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata

Part IV.  Laser Capture Microdissection

29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics
James L. Wittliff

30. Going in Vivo with Laser Microdissection
Anette Mayer, Monika Stich, Dieter Brocksch, Karin SchĂŒtze, and Georgia Lahr

31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections
Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco