Techniques in Confocal Microscopy book cover

Techniques in Confocal Microscopy

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

Audience
Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.

Paperback, 544 Pages

Published: June 2010

Imprint: Academic Press

ISBN: 978-0-12-384658-7

Contents

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    Part I. Practical Considerations and Equipment


    1. Theoretical Basis of Confocal Microscopy
    Robert H. Webb

    2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope
    Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong

    3. Equipment for Mass Storage and Processing of Data
    Guy Cox

    4. Antifading Agents for Confocal Fluorescence Microscopy
    Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh

    5. Mounting Techniques for Confocal Microscopy
    Manabu Kagayama and Yasuyuki Sasano

    6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy
    Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe
     
    7. Use of Confocal Microscopy to Investigate Cell Structure and Function
    Ghassan Bkaily, Danielle Jacques

    8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy
    Xuejun Sun


    Part II. Functional Approaches

    9. Volume Measurements in Confocal Microscopy
    Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

    10. Quantitation of Phagocytosis by Confocal Microscopy
    George F. Babcock and Chad T Robinson MS

    11. Receptor-Ligand Internalization
    Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti

    12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy
    David W. Piston and Susan M. Knobel

    13. Trafficking of the Androgen Receptor
    Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan

    14. Localization of Proteases and Peptide Receptors by Confocal Microscopy
    Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa

    15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
    Barry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton

    16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the Eye
    Barry R. Masters and Matthias Böhnke

    17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) Method
    Sylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel

    18. Identification of Viral Infection by Confocal Microscopy
    David N. Howell and Sara E. Miller

    19. Membrane Trafficking
    Sabine Kupzig, San San Lee, and George Banting


    III. Green Fluorescent Protein

    20. Monitoring of Protein Secretion with Green Fluorescent Protein
    Christoph Kaether and Hans-Hermann Gerdes

    21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters
    Xiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li

    22. Measuring Protein Degradation with Green Fluorescent Protein
    Stephen R. Cronin and Randolph Y. Hampton

    23. Studying Nuclear Receptors with Green Fluorescent Protein Fusions
    Gordon L. Hager

    24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein Conjugates
    Larry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron

    25. Fluorescent Proteins in Single- and Multicolor Flow Cytometry
    Lonnie Lybarger and Robert Chervenak

    26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in Neurons
    L. A. C. Blair, K. K.Bence, and J. Marshall

    27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis Oocytes
    Atshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba

    28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living Cells
    John J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki Ohata


    Part IV.  Laser Capture Microdissection

    29. Laser Capture Microdissection and Its Applications in Genomics and Proteomics
    James L. Wittliff

    30. Going in Vivo with Laser Microdissection
    Anette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr

    31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin Sections
    Douglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco