RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways book cover

RNA Turnover in Eukaryotes: Analysis of Specialized and Quality Control RNA Decay Pathways

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.

Audience
Biochemists and researchers in related life science disciplines.

Included in series
Methods in Enzymology

Hardbound, 464 Pages

Published: December 2008

Imprint: Academic Press

ISBN: 978-0-12-374584-2

Contents

  • Methods to Study No-Go Decay in Saccharomyces cerevisiaeCell-Cycle Regulation of Histone mRNA Degradation in Mammalian Cells: Role of Translation and Oligouridylation Assays of ARE-Mediated mRNA Decay in CellsEvaluating the Control of mRNA Decay in Fission YeastIn Vivo Analysis of the Decay of Transcripts Generated by Cytoplasmic RNA VirusesQualitative and quantitative assessment of the activity of the yeast NMD pathwayNonsence Mediated mRNA Decay in C. elegansIn vivo Analysis of Plant Nonsense-Mediated Decay. Estimating Nuclear mRNA Decay in S. CerevisiaeIdentification and Analysis of tRNAs that are Degraded in Saccharomyces cerevisiae Due to Lack of ModificationsAnalysis of Non-Functional rRNA Decay in Saccharomyces cerevisiae Identifying Substrates of mRNA Decay Factors by a Combined RNA Interference and DNA Microarray ApproachAnalysis of RNA-Protein Interactions Using a Yeast Three-Hybrid SystemCo-Immunoprecipitation Techniques for Assessing RNA-Protein Interactions in vivoHow to Define Targets for Small Guide RNAs in RNA Silencing: A Biochemical ApproachRapid mRNA Deadenylation and Decay Triggered by MicroRNAsA Method to Detect miRNA Targets Using miRNA as a Primer

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