RNA Interference


  • David Engelke, University of Michigan, Ann Arbor, U.S.A.
  • John Rossi, City of Hope's Beckman Research Institute, Duarte, California, U.S.A.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe), and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput strategies and prospects as a human therapy agent.
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Biochemists, cell biologists, molecular biologists, geneticists


Book information

  • Published: February 2005
  • ISBN: 978-0-12-182797-7

Table of Contents

Transgene-induced RNA Interference as a Tool for Plant Functional GenomicsConstructs and Methods for hairpin RNA-mediated Gene Silencing in PlantsRNA interference in Caenorhabditis elegansHigh-throughput RNAi screens in Drosophila tissue culture cellsMechanistic insights aid computational siRNA design Novel methods for expressing RNAi in human cellsDelivery of Small Interfering RNA to Mammalian Cells in Culture Using Cationic Lipid/Polymer-Based Transfection ReagentsThe subcellular distribution of siRNA: directed delivery through RNA polymerase III expression cassettes and localization using in situ hybridizationViral delivery of recombinant shRNAsDownregulation of cellular genes by PCR cassettes expressing short interfering RNAsUsing short hairpin RNA expression vectors to study mammalian neural developmentAnalysis of T-cell development using siRNA to knockdown protein expressionViral delivery of recombinant siRNA expression: lentivirusParadigms for Conditional Expression of RNAi Molecules for use Against Viral TargetsHigh throughput RNAi strategies for target discovery and validation using synthetic siRNAs: functional genomics investigations of biological pathwaysRNA interference in vivo: towards synthetic siRNA based therapeuticsLabeling and characterization of small RNAs associated with the RNAi effector complex, RITSRNAi spreading in C. elegansHuman Dicer: Purification, properties, and interaction with PAZ PIWI domain proteinsDelivery of siRNA and siRNA Expression Constructions to Adult Mammals Using Hydrodynamic Intravascular InjectionAnalysis of siRNA function in RNAi using Drosophila Embryo extracts and Schneider cellsUsing RNA polymerase II to transcribe artificial microRNAsAdeno-associated virus vectors for short hairpin RNA expression Simple, Robust Strategies for Generating ddRNAi Constructs