Proteomic and Metabolomic Approaches to Biomarker Discovery

Edited by

  • Haleem J Issaq, Ph.D., Laboratory of Proteomics and Analytical Technologies, SAIC/Frederick National Cancer Institute at Frederick, Frederick, MD, USA.
  • Timothy D. Veenstra, Ph.D., Mass Spectrometry Center and Laboratory of Proteomics and Analytical Technologies, SAIC/Frederick National Cancer Institute at Frederick, Frederick, MD, USA.

Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes.

Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution.  The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process.

A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.

View full description

Audience

Biochemists, analytical chemists, bioanalytical chemists, biologists, medical technicians, clinical technicians, researchers in pharmaceuticals, graduate students and all interested in the field of biomarkers research.

 

Book information

  • Published: June 2013
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-394446-7

Reviews

"Researchers in various medical and biological specialties focus mostly on preparing samples for proteomic and metabolomic studies, rather than on using the imaging equipment."--Reference and Research Book News, August 2013




Table of Contents

  1. Biomarker Discovery: Study Design and Execution
  2. Proteomic Technologies for Biomarker Discovery
  3. Sample Preparation for Proteomics
  4. Sample preparation For metabolomics
  5. SOP for serum and plasma
  6. Current NMR Strategies
  7. Data-independent Mass Spectrometry Extends Detectable Dynamic Range
  8. GC/MS
  9. LC/MS for: (a) Metabolomics
  10. CE/MS for (a) Proteomics (b) Metabolomics
  11. Gel electrophoresis
  12. The Role of Two-dimensional Gel Electrophoresis in Biomarker Discovery
  13. Affinity
  14. Asp selective Microwave-supported acid proteolysis
  15. Fractionation of complex mixtures
  16. Protein and Metabolite Identification
  17. Quantitative proteomics
  18. Quantitative analysis of metabolites in biological fluids
  19. Statistical & computational analysis
  20. Top-down Proteomics for Biomarker Discovery
  21. A role for protein-protein interaction networks in the identification and characterization of potential biomarkers
  22. Protein microarrays
  23. Autoantibodies as biomarkers
  24. MicroRNAs and Biomarker Discovery
  25. Tissue imaging for biomarker discovery
  26. MS approaches for biomarker verification
  27. MS metabolomic data handling for biomarker discovery
  28. Biomarker Validation