Proteomic and Metabolomic Approaches to Biomarker Discovery book cover

Proteomic and Metabolomic Approaches to Biomarker Discovery

Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes.

Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution.  The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process.

A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research.


Biochemists, analytical chemists, bioanalytical chemists, biologists, medical technicians, clinical technicians, researchers in pharmaceuticals, graduate students and all interested in the field of biomarkers research.

Hardbound, 488 Pages

Published: June 2013

Imprint: Academic Press

ISBN: 978-0-12-394446-7


  • "Researchers in various medical and biological specialties focus mostly on preparing samples for proteomic and metabolomic studies, rather than on using the imaging equipment."--Reference and Research Book News, August 2013


    1. Biomarker Discovery: Study Design and Execution
    2. Proteomic Technologies for Biomarker Discovery
    3. Sample Preparation for Proteomics
    4. Sample preparation For metabolomics
    5. SOP for serum and plasma
    6. Current NMR Strategies
    7. Data-independent Mass Spectrometry Extends Detectable Dynamic Range
    8. GC/MS
    9. LC/MS for: (a) Metabolomics
    10. CE/MS for (a) Proteomics (b) Metabolomics
    11. Gel electrophoresis
    12. The Role of Two-dimensional Gel Electrophoresis in Biomarker Discovery
    13. Affinity
    14. Asp selective Microwave-supported acid proteolysis
    15. Fractionation of complex mixtures
    16. Protein and Metabolite Identification
    17. Quantitative proteomics
    18. Quantitative analysis of metabolites in biological fluids
    19. Statistical & computational analysis
    20. Top-down Proteomics for Biomarker Discovery
    21. A role for protein-protein interaction networks in the identification and characterization of potential biomarkers
    22. Protein microarrays
    23. Autoantibodies as biomarkers
    24. MicroRNAs and Biomarker Discovery
    25. Tissue imaging for biomarker discovery
    26. MS approaches for biomarker verification
    27. MS metabolomic data handling for biomarker discovery
    28. Biomarker Validation


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