Principles of Cloning

Edited by

  • Jose Cibelli, Cellular Reprogramming Laboratory Michigan State University Ann Arbor, MI, USA
  • Robert Lanza, Advanced Cell Technology, MA, USA
  • Keith Campbell, The University of Nottingham, UK
  • Michael West, BioTime Inc., Alameda, CA, USA

Principles of Cloning is the first comprehensive book on animal cloning since the creation of Dolly. The contributing authors are the principal investigators on each of the animal species cloned to date, and are expertly qualified to present the state-of-the-art information in their respective areas. Editors Cibelli, Lanza and West garnered worldwide spotlight late in 2001 when their company, Advanced Cell Technology, announced the successful engineering of the world's first cloned human embryo. The trio was featured in the US News & World Report December 2001 cover story, "The First Human Clone." The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine.
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Bioengineers, biochemists, cell biologists, bone biologists, and geneticists.


Book information

  • Published: August 2002
  • ISBN: 978-0-12-174597-4

Table of Contents

List of Contributors.Preface. Introduction.Historical Perspective. PART ONE-BASIC BIOLOGICAL PROCESESActivation of Mammalian Oocytes. The Nucleus. Nuclear Reprogramming: Biological and Technological Constraints.Plasticity of somatic nucleus by epigenetic reprogramming via cell Hybridization. Cell Cycle. Determinants of Pluripotency in Mammals.Cloning and Aging.PART TWO-METHODSMicromanipulation Techniques for Cloning. Microinsemination and Nuclear Transfer with Male Germ Cells. Development of viable mammalian embryos in vitro: Evolution of sequential media.Genetic and Phenotypic Similarity Among Members of Mammalian Clonal Sets. Genetic Modification and Cloning in Mammals. Pregnancy and Neonatal Care of Clone Animals. Donor Cell Type and Cloning Efficiency in Mammals.PART THREE-CLONING BY SPECIESThe Cloning of Amphibians. Cloning of Fish. Cloning of Mice. Cloning of Rabbits. Nuclear Transfer in Swine. Cloning of Cattle. Cloning of Sheep. Cloning of Goats. PART FOUR-CURRENTLY SOUGHT AFTER SPECIESCloning of Endangered Species. Cloning of Rats. PART FIVE-NUCLEAR TRANSFER IN PRIMATESCloning in Non-Human Primates. PART SIX-APPLICATIONSNuclear Transfer for Stem Cells (CRNT). Current Research and Commercial Applications of Cloning Technology. Transgenic Cloned Goats and the Production of Therapeutic Proteins. PART SEVEN-ETHICAL AND LEGAL AFFAIRSEthical Implications of Cloning. FINAL REMARKSMammalian Cloning- Challenges for the Future.