Nucleosomes, Histones & Chromatin Part A

Edited by

  • Carl Wu, National Cancer Institute, Bethesda, MD
  • C. Allis, The Rockefeller University, New York, NY

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.
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Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

 

Book information

  • Published: August 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-391940-3


Table of Contents

1. Methods applied to the study of Protein Arginine Methylation

  Mark Bedford

2. TBC

  Mair Churchill

3. Genome-wide mapping of nucleosomes in budding yeast

  David J. Clark and Bruce Howard


4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology

  Wouter de Laat


5. TBC

  Josee Dostie


6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins

  John M. Denu


7. Peptide microarrays to interrogate the ‘histone code’

  Brian Strahl and Stephen M. Fuchs


8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS

  Nicole Francis


9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry

  Ben Garcia


10. Measuring nucleosome turnover dynamics genome-wide

  Steven Henikoff


11. Comparative mRNA expression-profiling of chromatin factors

  Frank Holstege


12. High-Throughput MAPit Single-Molecule Footprinting

  Michael P. Kadde


13. Genome-wide in vitro reconstitution

  Philipp Korber


14. Quantitating interactions of nuclear factors with chromatin

  Karolin Luger


15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex

  Gaku Mizuguchi


16. TBC

  Jennifer Ottesen


17. TBC

  B. Franklin Pugh