Nucleosomes, Histones & Chromatin Part A book cover

Nucleosomes, Histones & Chromatin Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

Audience
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 390 Pages

Published: August 2012

Imprint: Academic Press

ISBN: 978-0-12-391940-3

Contents

  • 1. Methods applied to the study of Protein Arginine Methylation

      Mark Bedford

    2. TBC

      Mair Churchill

    3. Genome-wide mapping of nucleosomes in budding yeast

      David J. Clark and Bruce Howard


    4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology

      Wouter de Laat


    5. TBC

      Josee Dostie


    6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins

      John M. Denu


    7. Peptide microarrays to interrogate the ‘histone code’

      Brian Strahl and Stephen M. Fuchs


    8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS

      Nicole Francis


    9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry

      Ben Garcia


    10. Measuring nucleosome turnover dynamics genome-wide

      Steven Henikoff


    11. Comparative mRNA expression-profiling of chromatin factors

      Frank Holstege


    12. High-Throughput MAPit Single-Molecule Footprinting

      Michael P. Kadde


    13. Genome-wide in vitro reconstitution

      Philipp Korber


    14. Quantitating interactions of nuclear factors with chromatin

      Karolin Luger


    15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex

      Gaku Mizuguchi


    16. TBC

      Jennifer Ottesen


    17. TBC

      B. Franklin Pugh

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