The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use

Volume 1

By

  • Elizabeth Harris, Duke University, Durham, NC, USA

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants.This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually.

Hardbound, 480 Pages

Published: November 2008

Imprint: Academic Press

ISBN: 978-0-12-370874-8

  • Vol 1 Introduction to C. reinhardtii and Its Laboratory Use The Genus Chlamydomonas; Cell Architecture; Cell Division; Motility and Behavior; The Sexual Cycle; The Life of an Acetate Flagellate; Organelle Heredity; Chlamydomonas in the Laboratory Vol 2 The Chlamydomonas in Plant Science Starch Metabolism; Glycerolipid Biosynthesis; Nitrogen Assimilation and Its Regulation; Amino Acid Metabolism ; Sulfur: From Acquisition to Assimilation; Phosphate Metabolism and Responses to Phosphorus Deficiency; Hydrogenases, Hydrogen Production, and Anoxia;The CO2-Concentrating Mechanism and Carbon Assimilation; Rubisco; Transition Metal Nutrition: A Balance Between Deficiency and Toxicity; Thioredoxins and Related Proteins; The Mitochondrial Genome; Oxidative Phosphorylation: Building Blocks and Related Components; Light-harvesting Proteins; Photosystem I; Photosystem II: A Structural Perspective; The Cytochrome b6f Complex; The CF0F1 ATPsynthase-ase Complex of Photosynthesis; Chaperones and Proteases; Biosynthesis of Chlorophylls and Hemes; Carotenoids; State Transitions; Photoprotection and High Light Responses; The Chloroplast Genome; Chloroplast Transcription; Chloroplast RNA splicing; Chloroplast RNA Processing and Stability; Protein Synthesis in the Chloroplast; The CES Process Vol 3 : Cell Motility and Behavior A Stroll through Time with Chlamydomonas; Basal Bodies and Associated Structures ; Deflagellation; Intraflagellar Transport; Flagellar Length Control; Axonemal Dyneins: Assembly, Structure and Force Generation; The Flagellar Radial Spokes; The Flagellar Central Pair Apparatus; The Regulation of Axonemal Bending; Microtubule-membrane Iinteractions in Chlamydomonas Flagella; The Chlamydomonas Flagellar Membrane and Its Dynamic Properties.; Flagellar adhesion, flagellar-generated signaling, and gamete fusion during mating; Sensory Photoreceptors and Light Control of Flagellar Activity; Mitosis and Cytokinesis; The Chlamydomonas flagellum as a Model for Human Ciliary Disease

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