Molecular Microbiology Laboratory book cover

Molecular Microbiology Laboratory

A Writing-Intensive Course

Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.

Audience
Appropriate for college sophomores through seniors in any of the life sciences, including microbiology, genetics, general biology, biochemistry, molecular biology, botany, zoology, etc. The experiments are also appropriate for properly equipped junior colleges and liberal arts institutions.

Paperback, 259 Pages

Published: August 2012

Imprint: Academic Press

ISBN: 978-0-12-397044-2

Reviews

  • "This book is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports."--Anticancer Research, August 2013


Contents

  • Introduction
    Experiment 1: Plasmid Purification and Restriction Mapping
    Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein
    Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
    Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities
    Appendix A: Sample Laboratory Reports
    Appendix B: Sample Proposals
    Appendix C: Sample Tests
    Appendix D: Guidance for Presentations
    Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991)
    Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997)
    Appendix G: Sequence Editing Tutorial
    Appendix H: Peak Scanner Tutorial
    Appendix I: Adding Size Standards to Peak Scanner
    Appendix J: PRIMER Tutorial
    Appendix K: Checklist for Grading Laboratory Reports
    Appendix L: Peer Review Checklist
    Appendix M: Career Advice
    Appendix N: Molecular Microbiology Laboratory Preparation Manual
    Bibliography
    Index

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