Molecular Microbiology Laboratory book cover

Molecular Microbiology Laboratory

A Writing-Intensive Course

Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.

Paperback, 259 Pages

Published: August 2012

Imprint: Academic Press

ISBN: 978-0-12-397044-2

Reviews

  • "This book is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports."--Anticancer Research, August 2013


Contents

  • Introduction
    Experiment 1: Plasmid Purification and Restriction Mapping
    Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein
    Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes
    Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities
    Appendix A: Sample Laboratory Reports
    Appendix B: Sample Proposals
    Appendix C: Sample Tests
    Appendix D: Guidance for Presentations
    Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991)
    Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997)
    Appendix G: Sequence Editing Tutorial
    Appendix H: Peak Scanner Tutorial
    Appendix I: Adding Size Standards to Peak Scanner
    Appendix J: PRIMER Tutorial
    Appendix K: Checklist for Grading Laboratory Reports
    Appendix L: Peer Review Checklist
    Appendix M: Career Advice
    Appendix N: Molecular Microbiology Laboratory Preparation Manual
    Bibliography
    Index

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