Molecular Evolution, Producing the Biochemical Data, Part B book cover

Molecular Evolution, Producing the Biochemical Data, Part B

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. Molecular Evolution Producing the Biochemical Data part B is a continuation of methods published in Part A (1993, volume 224). The work is a very methodological look at markers, templates, genomes, datasets and analyses used in studies of biological diversity.

Audience
Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Included in series
Methods in Enzymology

Hardbound, 896 Pages

Published: May 2005

Imprint: Academic Press

ISBN: 978-0-12-182800-4

Contents

  • Isolation of DNA from plants with large amounts of secondary metabolitesNucleic Acid Isolation from Environmental Aqueous Samples Nucleic acid isolation from ecological samples—vertebrate gut floraNucleic acid isolation from ecological samples — fungal associations, lichensNucleic acid isolation from ecological samples—fungal associations, mycorrhizaeNucleic acid isolation from ecological samples—animal scat and other associated materialsIsolation and analysis of DNA from archaeological, clinical and natural history specimensIsolation and characterization of proteins from archaeological and ancient specimensAnimal Phylogenomics: Multiple Interspecific Genome ComparisonsISSR techniques for evolutionary biologyUse of Amplified Fragment Length Polymorphism (AFLP) markers in surveys of vertebrate diversityUse of AFLP markers in surveys of arthropod diversityUse of AFLP markers in surveys of plant diversityIsolating microsatellite DNA lociUse of microsatellites for parentage and kinship analyses in animalsUse of capillary array electrophoresis single strand conformational polymorphism analysis to estimate genetic diversity of candidate genes in germplasm collectionsRibosomal RNA probes and microarrays: Their potential use in assessing microbial biodiversityThe role of geographic analyses in locating, understanding and using plant genetic diversityIn Situ Hybridization of Phytoplankton Using Fluorescently-Labelled rRNA ProbesSequencing and comparing whole mitochondrial genomes of animalsMethods for obtaining and analyzing whole chloroplast genome sequencesConstruction of Bacterial Artificial Chromosome libraries for use in phylogenetic studies Comparative EST analyses in plant systemsIsolation of genes from plant Y chromosomesPreparation of samples for comparative studies of plant chromosomes using in situ hybridization methodsPreparation of samples for comparative studies of arthropod chromosomes: visualization, in situ hybridization, and genome size estimationExperimental methods for assaying natural transformation and inferring horizontal gene transferUse of confocal microscopy in comparative studies of vertebrate morphology PrimerSelect: A transcriptome-wide oligonucleotide primer pair design program for kinetic RT-PCR-based transcript profilingDetecting differential expression of parental or progenitor alleles in genetic hybrids and allopolyploidsgenome-wide analysis of gene expression changes in polyploidsDesigning experiments using spotted microarrays to detect gene regulation differences within and among speciesMethods for studying the evolution of plant reproductive structures: Comparative gene expression techniquesDeveloping antibodies to synthetic peptides based on comparative DNA sequencing of multigene familiesApplication of ancestral protein reconstruction in understanding protein function: GFP-like proteinsAdvances in phylogeny reconstruction from gene order and content dataAnalytical methods for detecting paralogy in molecular datasetsAnalytical methods for studying the evolution of paralogs using duplicate gene data setsSupertree construction in the genomic ageMaximum-likelihood methods for phylogeny estimationContext Dependence and Co-Evolution among Amino Acid Resideue in Proteins

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