Methods of Adipose Tissue Biology Part A book cover

Methods of Adipose Tissue Biology Part A

Methods of Adipose Tissue Biology is a must-have for anyone interested in obesity or the physiology of white or brown adipose tissues. It contains state-of-the-art methods from researchers who are world leaders in this field. Detailed lab protocols include methods to visualize adipocytes and adipose tissues in humans and experimental models, converting stem cells into white and brown adipocytes in vitro, evaluating aspects of adipocyte metabolism, inducibly knocking out genes in adipose tissues, and evaluating transcriptional control of adipogenesis on a global scale.

Audience

Investigators and students whose research interests include the development, physiology, or pathophysiology of adipose tissues.

Included in series
Methods in Enzymology

Hardbound, 360 Pages

Published: February 2014

Imprint: Academic Press

ISBN: 978-0-12-411619-1

Contents

  • 1. Adipocyte-Specific Transgenic and Knockout Models
    Sona Kang, Xingxing Kong and Evan D. Rosen
    2. Imaging White Adipose Tissue With Confocal Microscopy
    Gabriel Martinez-Santibañez, Kae Won Cho and Carey N. Lumeng
    3. Isolation and Study of Adipocyte Precursors
    Christopher Church, Ryan Berry and Matthew S Rodeheffer
    4. Imaging of Adipose Tissue
    Ryan Berry, Christopher Church, Martin T. Gericke, Elise Jeffery, Laura Colman and Matthew S. Rodeheffer
    5. Adipose Tissue Angiogenesis Assay
    Raziel Rojas-Rodriguez, Olga Gealekman, Maxwell E. Kruse, Brittany Rosenthal, Kishore Rao, SoYun Min, Karl D. Bellve, Lawrence M. Lifshitz and Silvia Corvera
    6. Quantifying Size and Number of Adipocytes in Adipose Tissue
    Sebastian D. Parlee, Stephen I. Lentz, Hiroyuki Mori and Ormond A. MacDougald
    7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo
    Erica L. Scheller, Nancy Troiano, Joshua N. VanHoutan, Mary A. Bouxsein, Jackie, A. Fretz, Yougen Xi, Tracy Nelson, Griffin Katz, Ryan Berry, Chris D. Church, Casey R. Doucette, Matthew S. Rodeheffer, Ormond A. MacDougald, Clifford J. Rosen and Mark C. Horowitz
    8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)
    M. Borga, A.Virtanen Kirsi, T.Romu, O.Dahlqvist Leinhard, A.Persson, P.Nuutila and S. Enerbäck
    9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin
    M.F. Michelle Sim, Md Mesbah Uddin Talukder, Rowena J. Dennis, J. Michael Edwardson and Justin J. Rochford
    10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes
    Miwako Nishio and Kumiko Saeki
    11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue
    Cheryl H. Vaughan, Eleen Zarebidaki, J. Christopher Ehlen and Timothy J. Bartness
    12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion.
    Richard Sotornik and Jean-Luc Ardilouze
    13. Isolation and Quantitation of Adiponectin Higher Order Complexes
    Joseph M. Rutkowski and Philipp E. Scherer
    14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq
    Ronni Nielsen and Susanne Mandrup
    15. Analysis and Isolation of Adipocytes by Flow Cytometry
    Susan M. Majka, Heidi L. Miller, Karen M. Helm, Alistaire S. Acosta, Christine R. Childs, Raymond Kong and Dwight J. Klemm
    16. Flow Cytometry Analyses of Adipose Tissue Macrophages
    Kae Won Cho, David L. Morris and Carey N. Lumeng

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