Measuring Biological Responses with Automated Microscopy


  • James Inglese, National Institutes of Health, National Human Genome Research Institute, Bethesda, MD, U.S.A.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
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Biochmemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists


Book information

  • Published: October 2006
  • ISBN: 978-0-12-182819-6

Table of Contents

Dynamic GFP Sensors for High-Content Analysis of the Cell CycleHigh Content Fluorescence-based Screening for Epigenetic ModulatorsDevelopment of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged ProteinsThe Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan GPCRs by Arrestin RecruitmentHigh Content Screening of Known G Protein-Coupled Receptors by Arrestin TranslocationCell Imaging Assays for G protein-coupled Receptor Internalization: Application to High Throughput ScreeningHigh throughput confocal microscopy for beta arrestin-GFP translocation GPCR assays using the Evotec OperaG-protein coupled receptor internalization assays in the High Content Screening FormatScreening for Activators of the Wnt/Fzd Pathway by Automated Fluorescent MicroscopyA live-cell, image based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylationQuantitative single cell studies of nuclear receptor function using high-resolution HTM and image analysesTracking the motion of individual proteins with semiconductor quantum dotsDevelopment and Application of Automatic High Resolution Light Microscopy for Cell Based ScreensAdenoviral Sensors for High-Content Cellular AnalysisCell based assays in primary endothelial cells to study multiple steps in inflammationDevelopment and Implementation of Multiplexed Cell-Based Imaging AssaysHigh throughput screening for modulators of stem cell differentiationHigh Content Kinetic Calcium Imaging in Drug Sensitive and Drug Resistant Human Breast Cancer CellsMeasurement and Analysis of Calcium Signaling in Heterogeneous Cell CulturesMulipliex Analysis of Inflammatory Signalling Pathways using a High Content Imaging SystemGeneration and Characterization of a Stable MK2-EGFP Cell Line and Subswquent Development of a High Content Imaging Assay on the Cellomics ArrayScan® Platform to Screen for p38 MAPK InhibitorsDevelopment and implementation of three MAPK signaling pathway imaging assays to provide MAPK module selectivity profiling for kinase inhibitors; MK2-EGFP translocation, c-Jun & ERK activation.Assay Development and Case History of a 32K Biased Library High Content MK2-EGFP Translocation Screen to Identify p38 MAPK Inhibitors on the ArrayScan® 3.1 Imaging PlatformDevelopment and Implementation of a dual target MAPK high content assay (MK2-EGFP translocation & cJun activation) to provide LO support for kinase inhibitorsCompound classification using image-based cellular phenotypesHigh Content Screening: Emerging Hardware and Software TechnologiesAn Infrastructure for High Throughput Microscopy: Instrumentation, Informatics, and IntegrationProtein Translocation Assays: Key Tools for Accessing New Biological Information with High Throughput MicroscopyHigh-Content Screening of Functional Genomic LibrariesFluorescent protein-based cellular assays analyzed by laser scanning microplate cytometry in 1536-well plate format High-Throughput Measurements of Biochemical Responses Using the Plate::Vision™ Multimode 96 Mini-lens Array ReaderSystems Cell Biology Based on High Content ScreeningDigital Autofocus Methods for Automated MicroscopyFluorescence Lifetime Imagine Microscopy – Two-dimensional distribution measurement of fluorescence lifetime