Manual of Techniques in Invertebrate Pathology book cover

Manual of Techniques in Invertebrate Pathology

Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.

Audience

Insect pathologists; biological control and integrated pest management specialists; entomologists; marine biologists, microbiologists; nematologists; protozoologists; virologists; mycologists and biologists involved in basic and applied research on invertebrate pathogens

Paperback, 504 Pages

Published: April 2012

Imprint: Academic Press

ISBN: 978-0-12-386899-2

Reviews

  • listing in Society of Invertebrate Pathology Newsletter


Contents


  • Contribution

    Preface to first edition

    Preface to second edition

    Chapter I Initial handling and diagnosis of diseased invertebrates

        1 Introduction

        2 Collection of diseased invertebrates and entomopathogens

        3 Examination in the laboratory

        4 Safety considerations

        Acknowledgments

        References

    Chapter II Basic techniques in insect virology

        1 Introduction

        2 Brief overview of the different groups of insect viruses

        3 Identification

        4 Virus propagation

        5 Virus quantification

        6 Quantification of virus activity (virulence)

        7 Cell culture techniques

        8 Reverse genetics

        References

        Glossary

        Appendix: Recipes for stains

    Chapter III Isolation, culture, preservation, and identification of entomopathogenic bacteria of the Bacilli

        1 Introduction

        2 Isolation

        3 Cultivation

        4 Preservation

        5 Identification

        6 Concluding remarks

        Acknowledgments

        Disclaimer

        References

        Appendix

    Chapter IV Bioassay of bacterial entomopathogens against insect larvae

        1 Introduction

        2 Laboratory bioassay of Bacillus pathogens against lepidopteran larvae

        3 Bioassay of Bacillus thuringiensis against dipteran larvae

        4 Bioassay of Bacillus thuringiensis against other orders

        5 Bioassay of other entomopathogens

        6 Conclusions

        Acknowledgments

        References

        Appendix: artificial diet recipes

    Chapter V Bacteria for use against soil-inhabiting insects

        1 Introduction

        2 Isolation

        3 Identification

        4 Propagation and production

        5 Bioassay

        6 Preservation

        References

        Appendix 1

    Chapter VI Identification of entomopathogenic fungi

        1 Introduction

        2 Quick guide to light microscopy

        3 Key to major genera of fungal entomopathogens

        4 Major genera of fungal entomopathogens

        References

        Brief glossary of mycological terms

        Appendix: recipes of stains and reagents

    Chapter VII Laboratory techniques used for entomopathogenic fungi: Hypocreales

        1 Introduction

        2 Detection and characterization

        3 Inoculum propagation and preparation

        4 Quantification

        5 Infection and bioassay

        6 Assessment of environmental parameters

        Acknowledgments

        References

        Appendix

    Chapter VIII Mass production of entomopathogenic Hypocreales

        1 Introduction

        2 Culture maintenance

        3 Solid substrate production of aerial conidia

        4 Liquid fermentation production

        Disclaimer

        Acknowledgments

        References

        Appendix

    Chapter IX Methods for study of the Entomophthorales

        1 Introduction

        2 Collecting and preparing specimens

        3 Species identification

        4 Isolation

        5 In vitro culture and production

        6 In vivo culture and production

        7 Bioassays

        Acknowledgments

        References

        Appendix: media

    Chapter X Preservation of entomopathogenic fungal cultures

        1 Introduction

        2 Preservation at temperatures above freezing

        3 Preservation by freeze-drying (lyophilization)

        4 Preservation in a frozen state

        5 Record keeping for storage facilities

        6 Other less common preservation techniques

        References

    Chapter XI Research methods for entomopathogenic microsporidia and other protists

        1 Introduction

        2 Identification

        3 Techniques for managing spores

        4 Molecular and proteomic techniques

        5 Propagation and production

        6 Controlling infections in insect colonies

        7 Bioassays and experimental infections

        Acknowledgments

        References

    Chapter XII Nematode parasites, pathogens and associates of insects and invertebrates of economic importance

        1 Introduction

        2 Major groups of nematodes associated with insects and other invertebrates of economic importance

        3 Sampling techniques

        4 Diagnostic methods

        5 Staining methods

        6 Culturing methods

        7 Nematode quantification methods

        8 Preservation methods

        9 Shipping and handling of samples

        10 Bioassays with entomopathogenic nematodes and symbiotic bacteria

        Acknowledgments

        References

        Appendix I

        Appendix II

    Chapter XIII Pathogens and parasites of terrestrial molluscs

        1 Introduction

        2 General techniques for working with terrestrial molluscs

        3 Major groups of pathogens and parasites associated with terrestrial molluscs

        Acknowledgments

        References

        Appendix

    Chapter XIV Testing the pathogenicity and infectivity of entomopathogens to mammals

        1 Introduction

        2 Definition of infection

        3 Definition of persistence

        4 Mammalian safety tests and philosophy

        5 Animal procurement and housing

        6 Modified tier scheme

        7 Conclusion

        Acknowledgments

        References

    Chapter XV Complementary techniques: preparations of entomopathogens and diseased specimens for more detailed study using microscopy

        1 Introduction

        2 Light microscopy

        3 Electron microscopy

        Disclaimer

        Acknowledgments

        References

        Appendix

    Index




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