Manual of Techniques in Invertebrate Pathology book cover

Manual of Techniques in Invertebrate Pathology

The second edition of Manual of Techniques in Invertebrate Pathology is written by an international group of experts that contribute a broad array of techniques for the identification, isolation, culture, bioassay, propagation, and storage of the major groups of entomopathogens. The manual provides general and specific background to experienced insect pathologists, biologists, and entomologists who work with pathogen groups that are new to them. It is also useful as a laboratory manual for courses in insect pathology and biological control and related areas of study. Safety testing of entomopathogens in mammals and complementary techniques for the preparation of entomopathogens are included as well as broader methods for the study of specimens such as microscopy and molecular techniques. This manual concentrates primarily on practical step-by-step aspects of the techniques, but also provides the reader with a short history, rationale for usage, guides to supplemental literature, plus recipes for media, fixatives, and stains.


Insect pathologists; biological control and integrated pest management specialists; entomologists; marine biologists, microbiologists; nematologists; protozoologists; virologists; mycologists and biologists involved in basic and applied research on invertebrate pathogens

Paperback, 504 Pages

Published: April 2012

Imprint: Academic Press

ISBN: 978-0-12-386899-2


  • listing in Society of Invertebrate Pathology Newsletter


  • Manual of Techniques in Invertebrate Pathology, 2nd edition

    Lawrence A. Lacey, editor

    1. Initial Handling and Diagnosis of Diseased Invertebrates

    Lawrence A. Lacey and Leellen Solter

    2. Basic Techniques in Insect Virology

    Karolin E. Eberle, Jörg T. Wennmann, Regina G. Kleespiesand Johannes A. Jehle

    3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli.

    Tanja W. Fisher and Steven F. Garczynski

    4. Bioassay of Bacterial Entomopathogens Against Insect Larvae

    Maureen O’Callaghan, Travis R. Glare and Lawrence A. Lacey

    5. Bacteria for use Against Soil-Inhabitating Insects

    Albrecht M. Koppenhöfer, Trevor A. Jackson, and Michael G. Klein

    6. Identification of Entomopathogenic Fungi

    Richard A. Humber

    7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales

    G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel

    8. Mass Production of Entomopathogenic Hypocreales

    Stefan T. Jaronski and Mark A. Jackson

    9. Methods for the Study of Entomophthorales

    Ann E. Hajek, Bernard Papierok, and Jørgen Eilenberg

    10. Preservation of Entomopathogenic Fungal Cultures

    Richard A. Humber

    11. Research Methods for Entomopathogenic Microsporidia and Other Protists

    Leellen Solter, James Becnel, and Jiri Vávra

    12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance

    S. Patricia Stock and Heidi Goodrich-Blair

    13. Pathogens and Parasites of Terrestrial Molluscs

    Michael J. Wilson

    14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals

    Joel P. Siegel

    15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy

    James J. Becnel


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