Lipidomics and Bioactive Lipids: Lipids and Cell Signaling
- H. Alex Brown
This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.
Graduate students, researchers in academics and industry studying biochemistry.
Methods in Enzymology
Hardbound, 392 Pages
Published: November 2007
Imprint: Academic Press
- Chapter 1Phospholipase A1 assays using a radio-labeled substrate and mass spectrometryREI MORIKAWA, MASAFUMI TSUJIMOTO, HIROYUKI ARAI and JUNKEN AOKIChapter 2Real-time Cell Assays of Phospholipases A2 Using Fluorogenic PhospholipidsDebasis Manna, Wonhwa ChoChapter 3Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteinsDavid M. Lehmann, Chujun Yuan and Alan V. SmrckaChapter 4 Biochemical Analysis of Phospholipase D.H. Alex Brown, Lee G. Henage, Anita M. Preininger, Yun Xiang, John H. ExtonChapter 5Measurement of Autotaxin/Lysophospholipase D ActivityAndrew J. Morris and Susan S. SmythChapter 6Platelet-Activating FactorJohn S. Owen, Michael J. Thomas, and Robert L. WykleChapter 7Quantitative measurement of PtdIns(3,4,5)P3H. Guillou, L. Stephens, P.T. HawkinsChapter 8Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary LymphocytesAmber C. Donahue, Michael G. Kharas, and David A. FrumanChapter 9Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteinsYasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman, and Takeaki YokozekiChapter 10Biochemical Analysis of Inositol Phosphate KinasesJames C. Otto, Sashidhar Mulugu, Peter C. Fridy, Shean-Tai Chiou, Blaine N. Armbruster, Anthony A. Ribeiro and John D. YorkChapter 11Analysis of the phosphoinositides and their aqueous metabolitesChristopher P. Berrie, Cristiano Iurisci, Enza Piccolo, Renzo Bagnati and Daniela CordaChapter 12Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism.Stefka Spassieva, Jacek Bielawski, Viviana Anelli, and Lina M. ObeidChapter 13Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivoMichael Maceyka, Sheldon Milstien, Sarah SpiegelChapter 14A rapid and sensitive method to measure secretion of sphingosine-1-phosphatePoulami Mitra, Shawn G. Payne, Sheldon Milstien, Sarah SpiegelChapter 15Ceramide Kinase and Ceramide-1-PhosphateDayanjan S. Wijesinghe, Nadia F. Lamour, Antonio Gomez-Munoz, and Charles E. ChalfantChapter 16Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in CellsRichard M. Epand and Matthew K. TophamChapter 17Lipid Phosphate Phosphatases from Saccharomyces cerevisiaeGeorge M. Carman and Wen-I Wu