Laboratory Methods in Enzymology: Protein Part C book cover

Laboratory Methods in Enzymology: Protein Part C

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 296 Pages

Published: April 2014

Imprint: Academic Press

ISBN: 978-0-12-420119-4

Contents

  • TCA Precipitation
    Coimmunoprecipitation of proteins from yeast
    Coupling Antibody to Cyanogen Bromide-Activated Sepharose
    Analysis of Protein–Protein Interactions by Coimmunoprecipitation
    Use and Application of Hydrophobic Interaction Chromatography for Protein Purification
    Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins
    Salting out of proteins using ammonium sulfate precipitation
    Using ion exchange chromatography to purify a recombinantly expressed protein
    Gel filtration chromatography (Size exclusion chromatography) of proteins
    Expression and Purification of Membrane Proteins
    Explanatory Chapter: Choosing the right detergent
    One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
    Coomassie Blue Staining
    Silver Staining of SDS-polyacrylamide Gel
    Standard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA Binding
    Protein Filter Binding
    Explanatory Chapter: Troubleshooting recombinant protein expression: general
    Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble
    Western Blotting using Chemiluminescent Substrates

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