Laboratory Methods in Enzymology: Protein Part C book cover

Laboratory Methods in Enzymology: Protein Part C

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Hardbound, 296 Pages

Published: April 2014

Imprint: Academic Press

ISBN: 978-0-12-420119-4

Contents

  • TCA Precipitation
    Coimmunoprecipitation of proteins from yeast
    Coupling Antibody to Cyanogen Bromide-Activated Sepharose
    Analysis of Protein–Protein Interactions by Coimmunoprecipitation
    Use and Application of Hydrophobic Interaction Chromatography for Protein Purification
    Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins
    Salting out of proteins using ammonium sulfate precipitation
    Using ion exchange chromatography to purify a recombinantly expressed protein
    Gel filtration chromatography (Size exclusion chromatography) of proteins
    Expression and Purification of Membrane Proteins
    Explanatory Chapter: Choosing the right detergent
    One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
    Coomassie Blue Staining
    Silver Staining of SDS-polyacrylamide Gel
    Standard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel shift Assays for RNA and DNA Binding
    Protein Filter Binding
    Explanatory Chapter: Troubleshooting recombinant protein expression: general
    Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble
    Western Blotting using Chemiluminescent Substrates

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