Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate book cover

Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.

In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.

Audience

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Included in series
Methods in Enzymology

Hardbound, 408 Pages

Published: November 2013

Imprint: Academic Press

ISBN: 978-0-12-420067-8

Contents

  • Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies

    Storage of Bacteria and Yeast

    Cellular Fractionation - Mammalian Cells

    Cellular Fractionation - Yeast cells

    Yeast-Gene Replacement Using PCR Products

    Measuring RNAi knockdown using qPCR

    Recombineering: Using Drug Cassettes to Knock out Genes in vivo

    Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae

    Gene targeting and Site-specific recombination in Mouse ES Cells

    Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos

    Growth Media for E. coli

    Saccharomyces cerevisiae Growth Media

    Immunohistochemistry on Freely Floating Fixed Tissue Sections

    Preparation of Slides for Microscopy from Frozen Tissue Sections

    Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry

    Preparation of Cells for Microscopy using Cytospin

    Preparation of Cells for Microscopy using Chamber Slides and Coverslips

    Preparation of Cells for Microscopy using ‘Cell Blocks’

    Enzymatic deglycosylation of glycoproteins

    Preparation of fatty acid or phospholipid vesicles by thin-film rehydration

    Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder

    Preparation of fatty acid micelles

    Reverse-phase HPLC Analysis and Purification of Small Molecules

    Thin Layer Chromatography

    ATP and GTP hydrolysis assays (TLC)

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