Laboratory Methods in Cell Biology

Biochemistry and Cell Culture

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.
View full description

Audience

Researchers and students in cell, molecular and developmental biology.

 

Book information

  • Published: November 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-405914-6


Table of Contents

  1. Extraction/Characterization of DNA
    Leland J. Cseke
  2. Immunoprecipitation for Isolation of Protein Signaling Complexes from Native Tissues
    Rory Fisher
  3. Realtime PCR
    Yibing Jia
  4. Single Cell Gel Electrophoresis
    Andrew R. Collins
  5. High Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
    Mads Gabrielsen
  6. Difference Gel Electrophoresis
    Jonathan S. Minden
  7. Optimization of Cell Cycle Measurement by Time Lapse-Microscopy
    Gaspar Banfalvi
  8. Transfection
    Anja K. Bosserhoff
  9. Cell Enrichment
    Jeff Chalmers and Maciej Zborowski
  10. Magnetic Batch Separation
    Jeff Chalmers
  11. Lateral Flow Assays
    Paul Corstjens
  12. Measurement of Cell Regeneration
    Karen Echeverri
  13. DNA Damage Analysis in Cells
    Bevin P. Engelward
  14. Purification of Cardiac Cells
    Keiichi Fukuda and Fumiyuki Hattori
  15. Endothelial Cell Tube Formation in vitro assays
    Diego Guidolin
  16. Single-particle Analysis
    Slavica Jonic and Carlos Oscar Sanchez Sorzano
  17. Measurement of Cell Invasivness
    Gera Neufeld
  18. Techniques for Cell Sorting
    Yoon Ghil Park
  19. Stem Cells for Drug Discovery
    Lee Rubin
  20. Quantifying Apoptosis in 96-well Plates
    Yiqun Shellman
  21. Pulse Cytometry
    Lydia Sohn
  22. Cell Motility
    David Soll and Deborah Wessels
  23. Single Cell Deposition
    Yun Sun
  24. Enzyme Sensors for Living Cells
    Jenny Yang
  25. Myoblast purifying by magnetic-activated cell sorting (MACS)
    Yoon Ghil Park