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Laboratory Methods in Cell Biology

Biochemistry and Cell Culture

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.

Hardbound, 544 Pages

Published: November 2012

Imprint: Academic Press

ISBN: 978-0-12-405914-6

Contents

    1. Extraction/Characterization of DNA
      Leland J. Cseke
    2. Immunoprecipitation for Isolation of Protein Signaling Complexes from Native Tissues
      Rory Fisher
    3. Realtime PCR
      Yibing Jia
    4. Single Cell Gel Electrophoresis
      Andrew R. Collins
    5. High Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
      Mads Gabrielsen
    6. Difference Gel Electrophoresis
      Jonathan S. Minden
    7. Optimization of Cell Cycle Measurement by Time Lapse-Microscopy
      Gaspar Banfalvi
    8. Transfection
      Anja K. Bosserhoff
    9. Cell Enrichment
      Jeff Chalmers and Maciej Zborowski
    10. Magnetic Batch Separation
      Jeff Chalmers
    11. Lateral Flow Assays
      Paul Corstjens
    12. Measurement of Cell Regeneration
      Karen Echeverri
    13. DNA Damage Analysis in Cells
      Bevin P. Engelward
    14. Purification of Cardiac Cells
      Keiichi Fukuda and Fumiyuki Hattori
    15. Endothelial Cell Tube Formation in vitro assays
      Diego Guidolin
    16. Single-particle Analysis
      Slavica Jonic and Carlos Oscar Sanchez Sorzano
    17. Measurement of Cell Invasivness
      Gera Neufeld
    18. Techniques for Cell Sorting
      Yoon Ghil Park
    19. Stem Cells for Drug Discovery
      Lee Rubin
    20. Quantifying Apoptosis in 96-well Plates
      Yiqun Shellman
    21. Pulse Cytometry
      Lydia Sohn
    22. Cell Motility
      David Soll and Deborah Wessels
    23. Single Cell Deposition
      Yun Sun
    24. Enzyme Sensors for Living Cells
      Jenny Yang

    25. Myoblast purifying by magnetic-activated cell sorting (MACS)
      Yoon Ghil Park

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