Laboratory Methods in Cell Biology: Imaging

Methods in Cell Biology

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

Cell biology spans among the widest diversity of methods in the biological sciences.  From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.
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Audience

Researchers and students in cell, molecular and developmental biology

 

Book information

  • Published: January 2013
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-407239-8


Table of Contents

  1. Single Molecule FRET Microscopy
    Victoria Birkedal (nee Ciulin)
  2. Bioluminescent Imaging of Gene Expressions
    X. Chen
  3. Confocal Imaging
    Anda Cornea
  4. Electron Microscopy of Bone
    Vincent Everts
  5. Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments
    Rory Fisher
  6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions
    Changdeng Hu and Yutaka Kodama
  7. Histochemistry
    Lothar Jennes
  8. Live Imaging
    Naoto Kawakami
  9. Click Labeling for Single-Molecule FRET
    E.A. Lemke
  10. High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers
    Karen McLuskey & Mads Gabrielsen
  11. Correlation Microscopy
    Sasha Mironov
  12. Stereology of the Lung
    Matthias Ochs & Jan Philipp Schneider
  13. Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies.
    Francesco Pampaloni, Nariman Ansari & Ernst Stelzer
  14. Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy
    Tri Giang Phan
  15. Cryomicroscopy
    Ingeborg Schmidt-Krey
  16. In situ Hybridization
    Chung-Jui Tsai
  17. Imaging RNA
    Luis Vaca Dominguez