IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS book cover

IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS

LIVE CELL IMAGING OF CELLULAR ELEMENTS AND FUNCTIONS

This volume of Methods in Enzymology is the second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Audience
Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists

Included in series
Methods in Enzymology

Hardbound, 576 Pages

Published: February 2012

Imprint: Academic Press

ISBN: 978-0-12-388448-0

Contents

  • Part A: Organelles and Cellular Structure

    1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation
    2. Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

    3. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays
    4. Roy Edward

    5. Methods to measure actin treadmilling rate in dendritic spines
    6. Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

    7. Live Cell Imaging of Clathrin Coats
    8. Comert Kural and Tom Kirchhausen

    9. Imaging the Mitotic Spindle
    10. Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

    11. Modeling of spatial intracellular signaling events in neurons
    12. Wendy C. Wenderski and Susana R. Neves

    13. Imaging chromosome dynamics in meiosis in plants
    14. Moira J. Sheehan, and Wojciech P. Pawlowski

    15. Analysing lysosomes in live cells
    16. Paul R. Pryor

    17. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy
    18. Mario Brameshuber,and Gerhard J. Schütz

    19. Visualizing cardiac ion channel trafficking pathways
    20. James W. Smyth and Robin M. Shaw

    21. Live Cell Imaging of the Cytoskeleton
    22. Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

    23. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms
    24. Naoki Watanabe

    25. Analysis of cell dispersion and migration by video-microscopy
    26. Bonnomet Arnaud, Terryn Christine, Cutrona Jérôme, Jonquet Antoine, Birembaut Philippe, and Zahm Jean-Marie

      Part B: Molecules and Ions

    27. Real Time Qualitative and Quantitative Glut4 Translocation Assay
    28. Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat

    29. Advanced imaging of cellular signalling events
    30. JMarek Cebecauer, Jana Humpolíčková and Jerémie Rossy

    31. Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy
    32. Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami and Satyajit Mayor

    33. Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells
    34. Harsha E. Rajapakse and Lawrence W. Miller

    35. Imaging of corticosteroid receptors in live cells
    36. Mayumi Nishi

    37. Investigating second messenger signaling in vivo
    38. Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo

    39. Probes for Intracellular RNA Imaging In Live Cells
    40. Philip J. Santangelo, Eric Alonas, Jeenah Jung, Aaron W. Lifland, and Chiara Zurla

    41. Imaging the Glycome in Living Systems
    42. Boyangzi Li, Feiyan Mock and Peng Wu

    43. Intracellular Magnesium Detection by Fluorescent Indicators
    44. Valentina Trapani, Monika Schweigel-Röntgen, Achille Cittadini and Federica I. Wolf

    45. Illuminating mobile zinc with fluorescence: From cuvettes to live cells and tissues
    46. Zhen Huang and Stephen J. Lippard

    47. Quantitative fluorescent live cell imaging of intracellular Ca2+ and H+ ions in malaria parasites

              Petra Rohrbach

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