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IMAGING AND SPECTROSCOPIC ANALYSIS OF LIVING CELLS

LIVE CELL IMAGING OF CELLULAR ELEMENTS AND FUNCTIONS

This volume of Methods in Enzymology is the second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Included in series
Methods in Enzymology

Hardbound, 576 Pages

Published: February 2012

Imprint: Academic Press

ISBN: 978-0-12-388448-0

Contents

  • Part A: Organelles and Cellular Structure

    1. Monitoring Cytoskeletal Dynamics in Living Neurons using Fluorescence Photoactivation

      2. Anne Gauthier-Kemper, Carina Weissmann, Hans-Jürgen Reyher, and Roland Brandt

    2. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays

      3. Roy Edward

    3. Methods to measure actin treadmilling rate in dendritic spines

      4. Mikko Koskinen, Enni Bertling, and Pirta Hotulainen

    4. Live Cell Imaging of Clathrin Coats

      5. Comert Kural and Tom Kirchhausen

    5. Imaging the Mitotic Spindle

      6. Paul S. Maddox, Anne-Marie Ladouceur, Rajesh Ranjan, Jonas Dorn, Hery Ratsima, Damien D’Amours, and Amy S. Maddox

    6. Modeling of spatial intracellular signaling events in neurons

      7. Wendy C. Wenderski and Susana R. Neves

    7. Imaging chromosome dynamics in meiosis in plants

      8. Moira J. Sheehan, and Wojciech P. Pawlowski

    8. Analysing lysosomes in live cells

      9. Paul R. Pryor

    9. Detection and Quantification of Biomolecular Association in Living Cells using Single Molecule Microscopy

      10. Mario Brameshuber,and Gerhard J. Schütz

    10. Visualizing cardiac ion channel trafficking pathways

      11. James W. Smyth and Robin M. Shaw

    11. Live Cell Imaging of the Cytoskeleton

      12. Eve G. Stringham, Nancy Marcus-Gueret, Laura Ramsay and Kristopher L. Schmidt

    12. Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms

      13. Naoki Watanabe

    13. Analysis of cell dispersion and migration by video-microscopy

      14. Bonnomet Arnaud, Terryn Christine, Cutrona Jérôme, Jonquet Antoine, Birembaut Philippe, and Zahm Jean-Marie

      Part B: Molecules and Ions

    14. Real Time Qualitative and Quantitative Glut4 Translocation Assay

      15. Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat

    15. Advanced imaging of cellular signalling events

      16. JMarek Cebecauer, Jana Humpolícková and Jerémie Rossy

    16. Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy

      17. Subhasri Ghosh, Suvrajit Saha, Debanjan Goswami, Sameera Bilgrami and Satyajit Mayor

    17. Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells

      18. Harsha E. Rajapakse and Lawrence W. Miller

    18. Imaging of corticosteroid receptors in live cells

      19. Mayumi Nishi

    19. Investigating second messenger signaling in vivo

      20. Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo

    20. Probes for Intracellular RNA Imaging In Live Cells

      21. Philip J. Santangelo, Eric Alonas, Jeenah Jung, Aaron W. Lifland, and Chiara Zurla

    21. Imaging the Glycome in Living Systems

      22. Boyangzi Li, Feiyan Mock and Peng Wu

    22. Intracellular Magnesium Detection by Fluorescent Indicators

      23. Valentina Trapani, Monika Schweigel-Röntgen, Achille Cittadini and Federica I. Wolf

    23. Illuminating mobile zinc with fluorescence: From cuvettes to live cells and tissues

      24. Zhen Huang and Stephen J. Lippard

    24. Quantitative fluorescent live cell imaging of intracellular Ca2+ and H+ ions in malaria parasites

              Petra Rohrbach

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