Hydrogen Peroxide and Cell Signaling, Part A book cover

Hydrogen Peroxide and Cell Signaling, Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Included in series
Methods in Enzymology

Hardbound, 328 Pages

Published: July 2013

Imprint: Academic Press

ISBN: 978-0-12-405883-5


      1. Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light
        Fiona A. Summers, Baozhong Zhao, Douglas Ganini, and Ronald P. Mason
      2. Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems
        Vivian S. Lin, Bryan C. Dickinson and Christopher J. Chang
      3. Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe
        Nataliya M. Mishina, Kseniya N. Markvicheva, Dmitry S. Bilan, Mikhail Matlashov, David Liebl, Carsten Schultz, Sergey Lukyanov and Vsevolod V. Belousov
      4. In vivo Imaging of H2O2 Production in Drosophila
        Ana G. Barata and Tobias P. Dick
      5. Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging
        Lin Yuan, Weiying Lin, Sasa Zhu, Kaibo Zheng and Longwei He
      6. Electrochemical Biosensors for On-Chip Detection of Oxidative Stress
        James Enomoto, Zimple Matharu and Alexander Revzin
      7. Electrochemical Detection of H2O2 Formation in Isolated Mitochondria
        Stefania Rapino, Raluca Marcu, Francesco Paolucci and Marco Giorgio
      8. Detection of H2O2 by Fluorescence Correlation Spectroscopy
        Etsuro Ito, Satoshi Watabe, Mika Morikawa, Hiromi Kodama, Ryuichi Okada and Toshiaki Miura
      9. Real-Time Monitoring of Reactive Oxygen and Nitrogen Species in a multi-well plate using the diagnostic marker products of specific probes
        Jacek Zielonka, Joy Joseph, Adam Sikora and Balaraman Kalyanaraman
      10. H2O2 Delivery to Cells: Steady-State versus Bolus Addition
        H. Susana Marinho, Luísa Cyrne, Enrique Cadenas and Fernando Antunes
      11. Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling
        Nataliya M. Mishina, Kseniya N. Markvicheva, Arkady F. Fradkov, Elena V. Zagainova, Carsten Schultz, Sergey Lukyanov and Vsevolod V. Belousov
      12. The Determination aAnd Analysis oOf Site-Specific Rates oOf Mitochondrial Reactive Oxygen Species Production
        Casey L. Quinlan, Irina V. Perevoschikova, Renata L. S. Goncalves, Martin Hey-Mogensen and Martin D. Brand
      13. A Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging
      14. Ariel Kniss, Hang Lu, Dean P. Jones and Melissa L. Kemp

      15. Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.

      Guy Landau, Vamsi K. Kodali, Jyoti D. Malhotra and Randal J. Kaufman



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