Hydrogen Peroxide and Cell Signaling, Part A

Edited by

  • Lester Packer, Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA
  • Enrique Cadenas, Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.
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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.


Book information

  • Published: July 2013
  • ISBN: 978-0-12-405883-5

Table of Contents

    1. Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light
      Fiona A. Summers, Baozhong Zhao, Douglas Ganini, and Ronald P. Mason
    2. Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems
      Vivian S. Lin, Bryan C. Dickinson and Christopher J. Chang
    3. Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe
      Nataliya M. Mishina, Kseniya N. Markvicheva, Dmitry S. Bilan, Mikhail Matlashov, David Liebl, Carsten Schultz, Sergey Lukyanov and Vsevolod V. Belousov
    4. In vivo Imaging of H2O2 Production in Drosophila
      Ana G. Barata and Tobias P. Dick
    5. Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging
      Lin Yuan, Weiying Lin, Sasa Zhu, Kaibo Zheng and Longwei He
    6. Electrochemical Biosensors for On-Chip Detection of Oxidative Stress
      James Enomoto, Zimple Matharu and Alexander Revzin
    7. Electrochemical Detection of H2O2 Formation in Isolated Mitochondria
      Stefania Rapino, Raluca Marcu, Francesco Paolucci and Marco Giorgio
    8. Detection of H2O2 by Fluorescence Correlation Spectroscopy
      Etsuro Ito, Satoshi Watabe, Mika Morikawa, Hiromi Kodama, Ryuichi Okada and Toshiaki Miura
    9. Real-Time Monitoring of Reactive Oxygen and Nitrogen Species in a multi-well plate using the diagnostic marker products of specific probes
      Jacek Zielonka, Joy Joseph, Adam Sikora and Balaraman Kalyanaraman
    10. H2O2 Delivery to Cells: Steady-State versus Bolus Addition
      H. Susana Marinho, Luísa Cyrne, Enrique Cadenas and Fernando Antunes
    11. Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling
      Nataliya M. Mishina, Kseniya N. Markvicheva, Arkady F. Fradkov, Elena V. Zagainova, Carsten Schultz, Sergey Lukyanov and Vsevolod V. Belousov
    12. The Determination aAnd Analysis oOf Site-Specific Rates oOf Mitochondrial Reactive Oxygen Species Production
      Casey L. Quinlan, Irina V. Perevoschikova, Renata L. S. Goncalves, Martin Hey-Mogensen and Martin D. Brand
    13. A Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging
    14. Ariel Kniss, Hang Lu, Dean P. Jones and Melissa L. Kemp

    15. Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.

    Guy Landau, Vamsi K. Kodali, Jyoti D. Malhotra and Randal J. Kaufman