Human Stem Cell Manual
A Laboratory Guide
Edited by- Suzanne Peterson, The Scripps Research Institute, La Jolla, CA
- Jeanne Loring, The Burnham Institute, La Jolla, California, U.S.A.
- Robin Wesselschmidt
- Philip Schwartz
Audience
Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses.
Spiral Bound, 488 Pages
Published: June 2007
Imprint: Academic Press
ISBN: 978-0-12-370465-8
Contents
- Foreword by James BatteyForeword by Jeannie FontanaPrefacePart I: Basic methods: Maintenance of stem cell cultures1.Human Embryonic Stem Cell Culture 2.Feeder layers and feeder-free culture3.Mouse Embryonic Fibroblast Feeder Cells 4.Cryopreservation of hESC Part II: Basic methods: Characterization of stem cells5.Classical Cytogenetics: Karyotyping6.Spectral Karyotyping and Fluorescent in situ Hybridization 7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns8.FACS/Cell sorting9.Fluorescence Immunocytochemical Analysis of Stem Cells 10.Characterization of Stem Cells Using RT-PCR11.Gene Expression Profiling of Stem Cells by Microarray12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice 13.Generation of HESC-Derived Teratomas Part III: Differentiation of stem cells14.Embryoid Body Formation and Neuroepithelia Differentiation15.Motor Neuron and Dopamine Neuron Differentiation 16.Oligodendrocyte Differentiation from hESC 17.Cardiac 18.Hematopoietic Part IV: Genetic manipulation of stem cells19.Genetic Manipulation of hESC: Lentivirus Vectors 20.Methods developed for mouse ES cellsPart V: Advanced methods21.Derivation of human ES cells22.Neural Stem Cell Culture 23.Transplantation of stem cells24.In vitro fertilization25.Functional characterization-neural cellsPart VI: Practical issues in establishing a human ES cell laboratory26.Setting Up a Facility for hESC Research Part VII: Essays on stem cell biology 27.Intellectual Property: Owning the Stem Cell 28.Ethical Concerns for Stem Cell Research 29.Guidelines for hESC Research Oversight (ESCRO) Committees
