Hemoglobins, Part B: Biochemical and Analytical Methods

Edited by

  • Johannes Everse, School of Medicine, Texas Tech University Health Science Center, Lubbock, U.S.A.
  • Kim Vandegriff, University of California, Department of Medicine and Veterans Affairs Medical Center, San Diego, U.S.A.
  • Robert Winslow, University of California, Department of Medicine and Veterans Affairs Medical Center, San Diego, U.S.A.


  • John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
  • Melvin Simon, The Salk Institute, La Jolla, CA, USA

Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of Methods in Enzymology and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago.
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Biochemists, molecular biologists, cell biologists, physiolgists, pathologists, biophysicists, clinical chemists, industrial and academic researchers involved in the preparation of hemoglobin derivatives and in the preparationand analysis of recombinant proteins.


Book information

  • Published: March 1994
  • ISBN: 978-0-12-182132-6


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Table of Contents

Preparation, Purification, and Analysis of Human Hemoglobin:R.M. Winslow and K.W. Chapman, Pilot-Scale Preparation of Hemoglobin Solutions.B.J.-M. Thevenin and S.B. Shohet, Detection of Red Cell Membrane Components in Human Hemoglobin Preparations.B. Masala and L. Manca, Detection of Globin Chains by Reversed-Phase High-Performance Liquid Chromatography.P. Ferranti, A. Malorni, and P. Pucci, Structural Characterization of Hemoglobin Variants Using Capillary Electrophoresis and Fast Atom Bombardment Mass Spectrometry.M.P. Cohen and V.-Y. Wu, Purification of Glycated Hemoglobin.R.I. Roth and J. Levin, Measurement of Endotoxin Levels in Hemoglobin Preparations.Preparation and Characterization of Nonhuman Hemoglobins:H.H. Ruf, A.G. Altemaller, and K. Gersonde, Preparation and Characterization of Insect Hemoglobins from Chironomus thummi thummi.S.N. Vinogradov and P.K. Sharma, Preparation and Characterization of Invertebrate Globin Complexes.A. DeYoung, L.D. Kwiatkowski, and R.W. Noble, Fish Hemoglobins.C. Fronticelli and E. Bucci, Conformational and Functional Characteristics of Bovine Hemoglobin.Hemoglobin Hybrids and Model Hemes:E. Tsuchida and T. Komatsu, Synthetic Hemes. R.P. Roy and A.S. Acharya, Semisynthesis of Hemoglobin.A. Tsuneshige and T. Yonetani, Preparation of Mixed Metal Hybrids.Chemically Modified Hemoglobins:J.M. Manning, Preparation of Hemoglobin Derivatives Selectively or Randomly Modified at Amino Groups.M.J. Rao and A.S. Acharya, Amidation of Basic Carboxyl Groups of Hemoglobin.R.E. Benesch, Bis(pyridoxal) Polyphosphates as Specific Intramolecular Cross-Linking Agents for Hemoglobin.R.Y. Walder, M.E. Andracki, and J.A. Walder, Preparation of Intramolecularly Cross-Linked Hemoglobins.D.L. Currell, Derivatives of Hemoglobin Prepared by Reaction with Aryl or Alkyl Isothiocyanates.S.L. MacDonald and D.S. Pepper, Hemoglobin Polymerization.H. Xue and J. T.-F. Wong, Preparation of Conjugated Hemoglobins.R.T. Jones, Structural Characterization of Modified Hemoglobins.Recombinant Hemoglobin:T.-H. Jessen, N.H. Komiyama, J. Tame, J. Pagnier, D. Shih, B. Luisi, G. Fermi, and K. Nagai, Production of Human Hemoglobin in Escherichia coli Using Cleavable Fusion Protein Expression Vector.D. Looker, A.J. Mathews, J.O. Neway, and G.L. Stetler, Expression of Recombinant Human Hemoglobin in Escherichia coli.J.E. Ogden, R. Harris, and M.T. Wilson, Production of Recombinant Human Hemoglobin A in Saccharomyces cerevisiae.J.J.M. de Llano, O. Schneewind, G.L. Stetler, and J.M. Manning, Purification and Characterization of Recombinant Human Sickle Cell Hemoglobin Expressed in Yeast.M.P. Reilly, S.L. McCune, T.M. Ryan, T.M. Townes, M. Katsumata, and T. Asakura, Preparation of Recombinant Hemoglobin in Transgenic Mice.J.S. Logan and M.J. Martin, Transgenic Swine as Recombinant Production System for Human Hemoglobin.Hemoglobin Stability and Degradation:J.M. Rifkind, O. Abugo, A. Levy, and J. Heim, Detection, Formation, and Relevance of Hemichromes and Hemochromes.V.W. Macdonald, Measuring Relative Rates of Hemoglobin Oxidation and Denaturation.C. Giulivi and K.J.A. Davies, Hydrogen Peroxide-Mediated Ferrylhemoglobin Generation in Vitro and in Red Blood Cells.R.E. Benesch, The Stability of the Heme-Globin Linkage: Measurement of Heme Exchange.S.S. Panter, Release of Iron from Hemoglobin.K.W. Olsen, Thermal Denaturation Procedures for Hemoglobin.J. Everse, Photochemical Reduction of Methemoglobin and Methemoglobin Derivatives.E.L. McGown, M. Khan, and K.Dill, Regeneration of Functional Hemoglobin from Partially Oxidized Hemoglobin in the Presence of Molecular Hydrogen and a Multicomponent Redox Catalyst.Enzymatic Reactions Catalyzed by Hemoglobin:J. Everse, M.C. Johnson, and M.A. Marini, Peroxidative Activities of Hemoglobin and Hemoglobin Derivatives.L. Zilletti, M. Ciuffi, S. Franchi-Micheli, F. Fusi, G. Gentilini, G. Moneti, M. Valoti, and G.P. Sgaragli, Cyclooxygenase Activity of Hemoglobin.J.J. Mieyal and D.W. Starke, Hydroxylation and Dealkylation Reactions Catalyzed by Hemoglobin.G. Belvedere and M. Samaja, Oxidation of Olefins Catalyzed by Hemoglobin.Xenobiotic Adducts of Human Hemoglobin:S.R. Tannenbaum and P.L. Skipper, Quantitative Analysisof Hemoglobin-Xenobiotic Adducts.J.S. Wishnok, Quantitative Mass Spectrometry of Hemoglobin Adducts.P.L. Skipper and W.G. Stillwell, Aromatic Amine-Hemoglobin Adducts.M. Tirnqvist, Epoxide Adducts to N-Terminal Valine of Hemoglobin.S.S. Hecht, S.G. Carmella, and S.E. Murphy, Tobacco-Specific Nitrosamine-Hemoglobin Adducts.K. Taghizadeh and P.L. Skipper, Benzo[a]pyrene Diol Epoxide and Related Polynuclear Aromatic Hydrocarbon Adducts of Hemoglobin.B.W. Day and K. Singh, Analysis of Hemoglobin Adducts by Fluorescence Spectroscopy.Addendum:E. Tsuchida and T. Komatsu, Addendum to Article [11].Author Index.Subject Index.