G Protein Coupled Receptors

Modeling, Activation, Interactions and Virtual Screening

Edited by

  • P. Michael Conn, Texas Tech University Health Sciences Center, Lubbock, USA

      This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors, and includes chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.

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Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Book information

  • Published: February 2013
  • ISBN: 978-0-12-407865-9

Table of Contents

GPCR Modeling

  1. The Protein Local Optimization Program and G-Protein Coupled Receptors: Loop Restoration and Applications to Homology Modeling
  2. Dahlia A. Goldfeld and Richard A. Friesner

  3. Modeling Active GPCR Conformations
  4. Bruck Taddese, Lisa M Simpson, Ian D Wall, Frank E Blaney and Christopher A Reynolds

  5. Molecular Modeling of Adenosine Receptors
  6. Adriano Martinelli and Gabriella Ortore

  7. Predicting G Protein-Coupled Receptors Families using Different Physiochemical Properties and Pseudo Amino Acid Composition
  8. Zia-ur-Rehman, Muhammad Tayyeb Mirza, Asifullah Khan, Henri Xhaard

  9. Computational Design and Experimental Characterization of GPCR Segment Models
  10. Abby L. Parrill

    GPCR Interactions with Other Molecules and Cross-Talk

  11. G Protein-Coupled Receptor Interaction with Small GTPases
  12. Chunmin Dong and Guangyu Wu

  13. Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G Protein Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA)
  14. Annie Barbeau, Antoine G. Godin, Jody L. Swift, Yves De Koninck, Paul W. Wiseman and Jean-Martin Beaulieu

  15. NMR Methods for Detection of Small Molecule Binding to RGS4
  16. Andrew J. Storaska and Richard R. Neubig

  17. Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-Protein Regulatory Motif in the Intact Cell
  18. Sukru Sadik Oner, Joe B. Blumer and Stephen M. Lanier

  19. Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors
  20. Adam M. Knepp, Thomas P. Sakmar, and Thomas Huber

  21. cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors
  22. Jesper Mosolff Mathiesen, Line Vedel and Hans Bräuner-Osborne

  23. Rhodopsin-Lipid Interactions Studied by NMR
  24. Olivier Soubias and Klaus Gawrisch

    GPCR Bias

  25. Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity
  26. Kathryn M. Appleton, Mi-Hye Lee, Christian Alele, Christine Alele, Deirdre K. Luttrell, Yuri K. Peterson, Thomas A . Morinelli, and Louis M. Luttrell

    Virtual Screening

  27. Application of Monte Carlo-based Receptor-Ensemble Docking to Virtual Screening for GPCR Ligands
  28. Santiago Vilar and Stefano Costanzi

  29. From Heptahelical Bundle to Hits from the Haystack: Structure-Based Virtual Screening for GPCR Ligands
  30. A.J. Kooistra, L. Roumen, R. Leurs, I.J.P. de Esch, C. de Graaf

    GPCR Activation

  31. Kinetics and Dynamics in the G-Protein Coupled Receptor Signaling Cascade
  32. Jean-Pierre Vilardaga, Timothy N. Feinstein, Vanessa L. Wehbi, and Guillermo Romero

  33. Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors
  34. Joseph Goncalves, Markus Eilers, Kieron South, Chikwado A. Opefi, Philippe Laissue, Philip J. Reeves and Steven O. Smith

  35. Post-Translation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism
  36. Hui Zheng, Horace H. Loh and Ping-Yee Law

  37. Protein S-Nitrosylation Measurement

Yu Qin, Anindya Dey and Yehia Daaka