Essential Zebrafish Methods: Genetics and Genomics

Edited by

  • H. Detrich, III, Northeastern University, Boston, MA, USA
  • Leonard Zon, Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA
  • Monte Westerfield, Institute of Neuroscience, University of Oregon, Eugene, USA

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.
This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.
View full description


Researchers in cell, developmental, and molecular biology; genetics


Book information

  • Published: September 2009
  • ISBN: 978-0-12-375160-7

Table of Contents

1. Haploid Screens and Gamma-Ray Mutagenesis (Charline Walker)
2. Positional Cloning of Mutated Zebrafish Genes (Will Talbot)
3. The Zebrafish Genome (John Postlethwait)
4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish (Adam Amsterdam)
5. Genetic Screens for Maternal-Effect Mutations (Francisco Pelegri)
6. Behavior Screening Assays in Zebrafish (Herwig Baier)
7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish (Cecelia B. Moens)
8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element (Koichi Kawakami)9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish (Charles Lee)
10. The Zon Laboratory Guide to Positional Cloning in Zebrafish (Leonard I. Zon)
11. Sleeping Beauty Transposon for Efficient Gene Delivery (Stephen C. Ecker)
12. Transgene Manipulation in Zebrafish by Using Recombinases (Gary W. Stuart)
13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI (Joachim Wittbrodt)
14. Cloning Zebrafish by Nuclear Transfer (Shuo Lin)
15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening (Christine Thisse)
16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish (Bill Trevarrow)
17. Common Diseases of Laboratory Zebrafish (Jennifer L. Matthews)
18. Zebrafish Sperm Cryopreservation (Thomas Look)