Essential Zebrafish Methods: Cell and Developmental Biology

Edited by

  • Monte Westerfield, Institute of Neuroscience, University of Oregon, Eugene, USA
  • Leonard Zon, Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA
  • H. Detrich, III, Northeastern University, Boston, MA, USA

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.
This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade” to both experienced researchers and more junior members in the lab.
View full description


Researchers in cell, developmental, and molecular biology; genetics


Book information

  • Published: September 2009
  • ISBN: 978-0-12-374599-6

Table of Contents

Overview of the Zebrafish SystemH. William Detrich IIICell Cycles and Development in the Embryonic ZebrafishDonald A. KanePrimary Fibroblast Cell CultureBarry H. PawProduction of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)John H. PostlethwaitAnalysis of Protein and Gene ExpressionTrevor JowettAnalysis of Zebrafish Development Using Explant Culture AssaysHazel SiveConfocal Microscopic Analysis of Morphogenetic MovementsMark S. CooperCytoskeletal Dynamics of the Zebrafish EmbryoLilianna Solinica-KrezelHaploid Screens and Gamma-Ray MutagenesisCharline WalkerPositional Cloning of Mutated Zebrafish GenesWill TalbotThe Zebrafish GenomeJohn PostlethwaitAnalyzing Axon Guidance in the Zebrafish Retinotectal SystemChi-Bin ChienAnalysis of the Cell Cycle in Zebrafish EmbryosJames F. AmatrudaCellular Dissection of Zebrafish HematopoiesisDavid TraverCulture of Embryonic Stem Cell Lines from ZebrafishPaul Collodi NeurogenesisLaure Bally-CuifTime-Lapse Microscopy of Brain DevelopmentScott E. FraserDevelopment of the Peripheral Sympathetic Nervous System in ZebrafishPaul D. HenionApproaches to Study Neurogenesis in the Zebrafish Retina Jarema MalickiInstrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms David W. TankDevelopment of Cartilage and BoneThomas F. SchillingMorphogenesis of the Jaw: Development Beyond the EmbryoPamela C. YelickCardiac DevelopmentDidier Y. R. StainierZebrafish Kidney DevelopmentIain A. DrummondRetroviral-Mediated Insertional Mutogenesis in ZebrafishAdam Amsterdam Genetic Screens for Maternal-Effect MutationsFrancisco PelegriBehavior Screening Assays in ZebrafishHerwig BaierA High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in ZebrafishCecelia B. MoensTransgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable ElementKoichi KawakamiMolecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in ZebrafishCharles LeeThe Zon Laboratory Guide to Positional Cloning in ZebrafishLeonard I. ZonSleeping Beauty Transposon for Efficient Gene DeliveryStephen C. EckerTransgene Manipulation in Zebrafish by Using RecombinasesGary W. StuartHighly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceIJoachim WittbrodtCloning Zebrafish by Nuclear TransferShuo LinSpatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization ScreeningChristine ThisseGenetic Backgrounds, Standard Lines, and Husbandry of ZebrafishBill TrevarrowCommon Diseases of Laboratory ZebrafishJennifer L. MatthewsZebrafish Sperm CryopreservationThomas Look