DNA Repair, Part A book cover

DNA Repair, Part A

This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch repair, genetic recombination, and double strand break repair.

Audience
Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Included in series
Methods in Enzymology

Hardbound, 624 Pages

Published: April 2006

Imprint: Academic Press

ISBN: 978-0-12-182813-4

Contents

  • Table of ContentsChapter 1 Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivoChapter 2Oxidative DNA glycolases: Recipes from Cloning to CharacterizationChapter 3 Purification and Characterization of NEIL1 and NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized basesChapter 4Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-linksChapter 5Isolation and analyses of MutY and MutY Homologs (MYH)Chapter 6Use of yeast for detection of endogenous abasic legions, their source and their repairChapter 7Activities and Mechanism of Pol BetaChapter 8Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenasesChapter 9Purification and Characterization of DNA Photolyases Chapter 10Genetic and in vitro assays of DNA deaminationChapter 11The XPC complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repairChapter 12Purification and Characterization of E. coli and Human Nucleotide Excision RepairChapter 13 Assays for transcription elongation by RNA polymerase II using oligo (dC)-tailed template with single DNA damage Chapter 14In vivo assays for transcription-coupled repairChapter 15TFIIh enzymatic activities in transcription and nucleotide excision repairChapter 16An assay for studying ubiquitylation of RNA polymerase II and other proteins in crude yeast extractsChapter 17Analysis of the excision step in human DNA mismatch repairChapter 18Characterisation of the “mismatch repairosome” and its role in the processing of modified nucleosides in vitroChapter 19Analysis of DNA Mismatch Repair in Cellular Response to DNA DamageChapter 20Characterization of E. coli Translesion Synthesis Polymerases and Their Accessory FactorsChapter 21Measuring the Fidelity of Translesion DNA SynthesisChapter 22DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their FunctionChapter 23Purification and Characterization of Escherichia coli DNA Polymerase VChapter 24Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical CharacterizationChapter 25Localisation of Y-family polymerases and the DNA polymerase switch in mammalian cellsChapter 26Repair of DNA Double Strand Breaks – in vivo BiochemistryChapter 27Assays for nonhomologous end-joining in extractsChapter 28Purification and assays of Saccharomyces cerevisiae homologous recombination proteinsChapter 29Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopyChapter 30Synthetic Junctions as Tools to Identify and Characterize Holliday Junction ResolvasesChapter 31In Vitro Nonhomologous End Joining AssayChapter 32The RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexesChapter 33Purification and Biochemical Characterization of ATM and MRN

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