Cytometry: New Developments, 75

  • Zbigniew Darzynkiewicz, Brander Cancer Research Institute at New York Medical College, Valhalla, U.S.A.
    • Mario Roederer, Immuno-Technology Section and Flow Cytometry Core, Vaccine Research Center, NIAD, NIH, Bethesda, MD, U.S.A.
      • Hans Tanke, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands


      Researchers in the fields of cell and molecular biology, immunology, and cytogenetics.


Book information

  • Published: November 2004
  • ISBN: 978-0-12-564170-8

Table of Contents

Section I: Intrumentation/Fluorochromes: Optimization of Emission Optics for Multicolog Flow Cytometry; Two-photon Tissue Cytometry; Confocal Microscopy: Quantitative Analytical Capabilities; Surface Plasmon Coupled Emission - New Technology for Studying Molecular Processes; Cytometry of Fluorescence Resonance Energy Transfer (FRET); The Rainbow of Fluorescent Proteins; Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates; Next Generation Laser Scanning Cytometry. Section II: General Techniques: Biohazard Sorting; Guidelines for the Presentation of Flow Cytometric Data; Mechanism of Antitumor Drug Action Assessed by Cytometry; Cytometric Methods to Detect Apoptosis; Real-time Analysis of Apoptosis in-vivo; Detection of DNA Damage in Individual Cells by Analysis of Histone H2AX Phosphorylation; Cytometry of Freshwater Phytoplankton; Multiplexed Microsphere Assays (MMAs) for Protein and DNA Binding Reactions. Section III: Immunology/T-Cell Responses: Flow Cytometry Applications of MHC Tetramers; The use of Peptides and Peptide Libraries as T-cell Stimulants in Flow-Cytometric Studies; Flow Cytometric Analysis of Human Antigen-Specific T-cell Proliferation; Detection of T-cell Degranulation: CD107a and b; T-cell Responses to Cancer. Section IV: Multi-Color Immunophenotyping: Multi-color Flow Cytometric Analysis of T Lymphocytes in SIV-Infected Rhesus Macaques; Multi-color Immunophenotyping-Human Immune System Hemopoiesis; Multi-color Immunophenotyping - Human Mature Immune System; Differential Diagnosis of T-cell Lymphoproliferative Disorders by Flow Cytometry Multi-color Immunophenotyping; Isolation and Immunophenotyping of Human and Rhesus Macaque Dendritic Cells; B-cell Immunophenotyping. Section V: Other Topics in Immunology: Flow Cytometry Immunophenotypic Characteristics of Moncytic Population in Acute Moncytic Leukemia (AML-M5), Acute Myelomonocytic Leukemia (AML-M4) and Chronic Myelomonocytic Leukemia (CMML); Phagocyte Function; Neutralizing Antibody Quantification by Flow Cytometry. Section VI: Cytogenetics: Telomere Length Measurements Using Fluorescence in situ Hybridization and Flow Cytometry (Flow-FISH); Detecting Copy Number Changes in Genomic DNA - MAPH and MLPA; Genomic Array Technology; In situ Analyses of Individual Molecules and Complexes of DNA, RNA, and Protein with Padlock and Proximity Probes; Use of Subtelomeric Probes to Study Mental Retardation.