Cytometry: New Developments, 75
- Zbigniew Darzynkiewicz, Brander Cancer Research Institute at New York Medical College, Valhalla, U.S.A.
- Mario Roederer, Immuno-Technology Section and Flow Cytometry Core, Vaccine Research Center, NIAD, NIH, Bethesda, MD, U.S.A.
- Hans Tanke, Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands
Audience
Researchers in the fields of cell and molecular biology, immunology, and cytogenetics.
Included in series
Methods in Cell Biology
Methods in Cell Biology
Hardbound, 920 pages
Published: November 2004
Imprint: Academic Press
ISBN: 978-0-12-564170-8
Contents
- Section I: Intrumentation/Fluorochromes: Optimization of Emission Optics for Multicolog Flow Cytometry; Two-photon Tissue Cytometry; Confocal Microscopy: Quantitative Analytical Capabilities; Surface Plasmon Coupled Emission - New Technology for Studying Molecular Processes; Cytometry of Fluorescence Resonance Energy Transfer (FRET); The Rainbow of Fluorescent Proteins; Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates; Next Generation Laser Scanning Cytometry. Section II: General Techniques: Biohazard Sorting; Guidelines for the Presentation of Flow Cytometric Data; Mechanism of Antitumor Drug Action Assessed by Cytometry; Cytometric Methods to Detect Apoptosis; Real-time Analysis of Apoptosis in-vivo; Detection of DNA Damage in Individual Cells by Analysis of Histone H2AX Phosphorylation; Cytometry of Freshwater Phytoplankton; Multiplexed Microsphere Assays (MMAs) for Protein and DNA Binding Reactions. Section III: Immunology/T-Cell Responses: Flow Cytometry Applications of MHC Tetramers; The use of Peptides and Peptide Libraries as T-cell Stimulants in Flow-Cytometric Studies; Flow Cytometric Analysis of Human Antigen-Specific T-cell Proliferation; Detection of T-cell Degranulation: CD107a and b; T-cell Responses to Cancer. Section IV: Multi-Color Immunophenotyping: Multi-color Flow Cytometric Analysis of T Lymphocytes in SIV-Infected Rhesus Macaques; Multi-color Immunophenotyping-Human Immune System Hemopoiesis; Multi-color Immunophenotyping - Human Mature Immune System; Differential Diagnosis of T-cell Lymphoproliferative Disorders by Flow Cytometry Multi-color Immunophenotyping; Isolation and Immunophenotyping of Human and Rhesus Macaque Dendritic Cells; B-cell Immunophenotyping. Section V: Other Topics in Immunology: Flow Cytometry Immunophenotypic Characteristics of Moncytic Population in Acute Moncytic Leukemia (AML-M5), Acute Myelomonocytic Leukemia (AML-M4) and Chronic Myelomonocytic Leukemia (CMML); Phagocyte Function; Neutralizing Antibody Quantification by Flow Cytometry. Section VI: Cytogenetics: Telomere Length Measurements Using Fluorescence in situ Hybridization and Flow Cytometry (Flow-FISH); Detecting Copy Number Changes in Genomic DNA - MAPH and MLPA; Genomic Array Technology; In situ Analyses of Individual Molecules and Complexes of DNA, RNA, and Protein with Padlock and Proximity Probes; Use of Subtelomeric Probes to Study Mental Retardation.
