Correlative Light and Electron MIcroscopy book cover

Correlative Light and Electron MIcroscopy

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.


Researchers and students in cell, molecular and developmental biology

Included in series
Methods in Cell Biology

Hardbound, 460 Pages

Published: August 2012

Imprint: Academic Press

ISBN: 978-0-12-416026-2


    1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    2. K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson & F. Braet

    3. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy.
    4. Roman S. Polishchuk, Elena V. Polishchuk, and Alberto Luini

    5. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    6. Toshihiro Takizawa and John M. Robinson

    7. Correlative Light and Electron Microscopy in Parasite Research
    8. Céline Loussert, Claire-Lise Forestier and Bruno M. Humbel

    9. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    10. Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, and Thomas Kurth

    11. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    12. Katia Cortese, Giuseppe Vicidomini, MariaCristina Gagliani1, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

    13. Correlative Light and Electron Microscopy of GFP
    14. Markus Grabenbauer

    15. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    16. Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu and Gina E. Sosinsky

    17. Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!
    18. Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan & Ben N. G. Giepmans

    19. Capturing Endocytic Segregation Events with HPF-CLEM
    20. Edward Brown, Jan van Weering, Thom Sharp, Judith Mantell and Paul Verkade

    21. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
    22. Irina Kolotuev, Daniel J Bumbarger, Michel Labouesse and Yannick Schwab

    23. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
    24. Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert

    25. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
    26. Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen and John A. G. Briggs

    27. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
    28. Alexander Rigort, Elizabeth Villa, Felix J. B. Bäuerlein and Jürgen M. Plitzko

    29. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
    30. Shigeki Watanabe and Erik M. Jorgensen

    31. Atmospheric Scanning Electron Microscope for Correlative Microscopy
    32. Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood and Peter J. O'Toole

    33. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
    34. Miriam Lucas, Maja Günthert, Philippe Gasser, Falk Lucas and Roger Wepf

    35. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

    Andrew J. Bushby, Giovanni Mariggi, Hannah E.J. Armer and Lucy M. Collinson


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