Correlative Light and Electron MIcroscopy

Edited by

  • Thomas Mueller-Reichert, Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
  • Paul Verkade, Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology & Pharmacology, University of Bristol, Bristol, UK

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
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Audience

Researchers and students in cell, molecular and developmental biology

 

Book information

  • Published: August 2012
  • Imprint: ACADEMIC PRESS
  • ISBN: 978-0-12-416026-2


Table of Contents

  1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
  2. K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson & F. Braet

  3. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy.
  4. Roman S. Polishchuk, Elena V. Polishchuk, and Alberto Luini

  5. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
  6. Toshihiro Takizawa and John M. Robinson

  7. Correlative Light and Electron Microscopy in Parasite Research
  8. Céline Loussert, Claire-Lise Forestier and Bruno M. Humbel

  9. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
  10. Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, and Thomas Kurth

  11. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
  12. Katia Cortese, Giuseppe Vicidomini, MariaCristina Gagliani1, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

  13. Correlative Light and Electron Microscopy of GFP
  14. Markus Grabenbauer

  15. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
  16. Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu and Gina E. Sosinsky

  17. Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!
  18. Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan & Ben N. G. Giepmans

  19. Capturing Endocytic Segregation Events with HPF-CLEM
  20. Edward Brown, Jan van Weering, Thom Sharp, Judith Mantell and Paul Verkade

  21. Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms
  22. Irina Kolotuev, Daniel J Bumbarger, Michel Labouesse and Yannick Schwab

  23. Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo
  24. Ina Woog, Silke White, Mandy Büchner, Martin Srayko and Thomas Müller-Reichert

  25. Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers
  26. Wanda Kukulski, Martin Schorb, Sonja Welsch, Andrea Picco, Marko Kaksonen and John A. G. Briggs

  27. Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining
  28. Alexander Rigort, Elizabeth Villa, Felix J. B. Bäuerlein and Jürgen M. Plitzko

  29. Visualizing Proteins in Electron Micrographs at Nanometer Resolution
  30. Shigeki Watanabe and Erik M. Jorgensen

  31. Atmospheric Scanning Electron Microscope for Correlative Microscopy
  32. Ian E.G. Morrison, Clare L. Dennison, Hidetoshi Nishiyama, Mitsuo Suga, Chikara Sato, Andrew Yarwood and Peter J. O'Toole

  33. Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures
  34. Miriam Lucas, Maja Günthert, Philippe Gasser, Falk Lucas and Roger Wepf

  35. Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms

Andrew J. Bushby, Giovanni Mariggi, Hannah E.J. Armer and Lucy M. Collinson