Correlative Light and Electron MIcroscopy
- Thomas Mueller-Reichert, Core Facility Cellular Imaging, Faculty of Medicine Carl Gustav Carus, Dresden, Germany
- Paul Verkade, Wolfson Bioimaging Facility, Schools of Biochemistry and Physiology & Pharmacology, University of Bristol, Bristol, UK
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
Researchers and students in cell, molecular and developmental biology