Colloidal Gold, 3 book cover

Colloidal Gold, 3

Principles, Methods, and Applications

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Audience
Researchers, technicians, teachers, and students in cell and developmental biology, immunocytochemistry, histochemistry, and electron microscopy; also anatomy, pathology, and microbiology. Academic and industrial institutional markets. Training courses and workshops on colloidal gold techniques.

Hardbound, 421 pages

Published: December 1990

Imprint: Academic Press

ISBN: 978-0-12-333929-4

Reviews

  • "The chapters in these volumes are a measure of a healthy field of research and its applications."
    --ELECTRON MICROSCOPY REVIEW


    "If you need to localise something within a cell, the colloidal gold series can probably tell you how to go about it."
    --EUROPEAN JOURNAL OF PROTISTOL
    "This treatise is timely and extremely helpful for the scientist interested in applying the colloidal gold methodology for a particular research problem...The present volumes elegantly cover broad research applications that reach beyond the traditional field of microscopy in cell biology and extend into the field of molecular biology... An attempt has been made to include a distinguished group of experts who are the originators of the techniques; many continue to play an active role in the technical development. The authors are from diverse fields, so that these books will be attractive to a wide audience including cell biologists, pathologists, virologists, and developmental and molecular biologists. The printing of the hardbound books is of prime quality, with excellent reproductions of the well-chosen micrographs... The text, printed on glossy paper, is extremely easy to read; diagrams, drawings, and tables throughout the two volumes are particularly pertinent in illustrating the principles and steps of many of the procedures. Although each chapter is written by a different author, all consistently follow a well organized pattern. The chapters begin with an introduction and historical background, then follow with preparation of probes and specimens, principles and descriptions of the methods for labeling, descriptions of the experimental results with a review of the literature, conclusions, and a list of citations to many original references. A particularly valuable part of each chapter is the assessment of the advantages, limitation, and pitfalls of a given methodology... These two volumes are a mix of reference book, student textbook, and cookbook. On the whole, the descriptions of methods are thorough and stimulating, and will be most useful to the experienced reader seeking to select the procedure most suited to a particular research problem. The in-depth account of the methodology and the extensive references provided will be of great help to those inter
    --EMSA BULLETIN

Contents

  • Some Basic Concepts for the Choice of Methods. Techniques for the Production of Monoclonal and Polyclonal Antibodies. Lowicryl Resins. Polar and Apolar Lowicryl Resin for Immunolabeling. Comparable Yield of Immunolabel by Resin Sections and Thawed Cryosectins. Colloidal Gold Labeling for Determining Cell Surface Area. Quantitation of Colloidal Gold by Electron Microscopy. Simultaneous Demonstration of Antigens on Outer and Protoplasmic Surfaces of the Plasma Membrane by Replica Immunocytochemistry. Double Labeling of Antigenic Sites on Cell Surfaces Imaged with Backscattered Electrons. Location and Identification of Colloidal Gold Particles with an Energy Dispersive Analyzer. Use of Dinitrophenol IgG Conjugates: Immunogold Labeling of Cellular Antigens on Thin Sections of Osmicated and Epon-Embedded Specimens. Preparation and Application of Insulin-Gold Complex. Microinjection of Colloidal Gold. Combined Diaminobenzidine-Colloidal Gold Staining. Colloidal Gold Techniques and the Immunocytochemistry of Antigen-Antibody Reactions at Interfaces. Colloidal Gold Labeling for Diagnostic Pathology. Immunogold Silver Staining Using Microwave Irradiation. A Correlative Approach to Colloidal Gold Labeling with Video-Enhanced Light Microscopy, Low Voltage Scanning Electron Microscopy, and High Voltage Electron Microscopy.

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