Biophysical Tools for Biologists

In Vitro Techniques

Edited by

  • John Correia, University of Mississippi Medical Center, Jackson, USA
  • H. Detrich, III, Northeastern University, Boston, MA, USA

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
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Cell biologists, biophysicists, pharmacologists, and molecular biologists.


Book information

  • Published: November 2007
  • ISBN: 978-0-12-372520-2

Table of Contents

Part I: History and ReflectionsSection 1. Solution Methods 1. Binding: A Polemic and Rough Guide Nichola C. Garbett, Jonathan B. Chaires2. Linked Equilibria in Regulation of Transcription Initiation Dorothy Beckett3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular InteractionsFarial A. Tanious, Binh Nguyen, and W. David Wilson4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic InteractionsMatthew W. Freyer and Edwin A. Lewis 5. Differential Scanning CalorimetryCharles H. Spink6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation EquilibriumTom Laue, Tom Moody, James Cole, and Jeff Lary7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer MethodsSusan Bane and Natasha Shanker 9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA InteractionsVince J. LiCata and Andy J. Wowor10. Circular Dichroism and Its Application to the Study of BiomoleculesStephen R. Martin and Maria J. Schilstra11. Folding and StabilityTimothy O. Street, Naomi Courtemanche and Doug Barrick12. Hydrodynamic shape modelling of analytical ultracentrifugation dataOlwyn Byron13. X-ray and neutron scattering data and their constrained molecular modellingStephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado 14. Structural Investigations into Microtubule-MAP ComplexesAndreas Hoenger and Heinz Gross15. Rapid Kinetic TechniquesJohn F. Eccleston, Stephen Martin, and Maria J. Schilstra16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model ExampleGeorge J. Turner17. Quantifying DNA-protein interactions by single molecule stretchingMark C. Williams, Ioulia Rouzina, and Richard L. Karpel18. Isotopomer-based metabolomic analysis by NMR and mass spectrometryAndrew N Lane, Teresa W-M. Fan, and Richard M. Higashi19. Following molecular transitions with single residue spatial and millisecond time resolutionInna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz 20. Methods and applications of site-directed spin labeling EPR spectroscopyCandice S. Klug and Jimmy B. Feix21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactionsHacène Boukari and Dan L. Sackett22. Practical Guide to OsmolytesJorg Rosgen and Daniel Harries23. Stupid StatisticsJoel Tellinghuisen24. Nonlinear Least Squares Fitting MethodsMichael L. Johnson25. Methods for Simulating the Dynamics of Complex Biological ProcessesMaria J Schilstra, Stephen R. Martin, and Sarah M. Keating26. Computational Methods for Biomolecular ElectrostaticsFeng Dong, Brett Olsen and Nathan A. Baker27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stabilitySteven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser28. Molecular Modeling of Cytoskeletal ProteinsXiange Zheng and David Sept29. Mathematical Modeling of Cell MotilityAnders E. Carlsson and David Sept