Bioconjugate Techniques


  • Greg T. Hermanson, Director of Technology, Thermo Fisher Scientific, Rockford, IL, USA

Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.
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Book information

  • Published: August 2013
  • ISBN: 978-0-12-382239-0

Table of Contents

  1. Introduction to Bioconjugation
  2. Functional Targets for Bioconjugation
  3. The Reactions of Bioconjugation
  4. Zero-Length Crosslinkers
  5. Homobifunctional Crosslinkers
  6. Heterobifunctional Crosslinkers
  7. Trifunctional Crosslinkers
  8. Dendrimers and Dendrons
  9. Cross-Bridges and Cleavable Reagent Systems
  10. Fluorescent Probes
  11. (Strept)avidin - Biotin Systems
  12. Isotopic Labeling Techniques
  13. Silane Coupling Agents
  14. Microparticles and Nanoparticles
  15. Immobilization of Ligands on Chromatography Supports
  16. Buckyballs, Fullerenes, and Carbon Nanotubes
  17. Chemoselective Ligation; Bioorthogonal Reagents
  18. PEGylation & Synthetic Polymer Modification
  19. Vaccine and Immunogen Conjugates
  20. Antibody Modification and Conjugation
  21. Liposome Conjugates and Derivatives
  22. Enzyme Modification and Conjugation
  23. Nucleic Acid and Oligonucleotide Modification and Conjugation
  24. Bioconjugation for the Study of Protein Interactions