Bioconjugate Techniques


  • Greg Hermanson, Director of Technology, Thermo Fisher Scientific, Rockford, IL USA


Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology


Book information

  • Published: April 2008
  • ISBN: 978-0-12-370501-3

Table of Contents

PART IBioconjugate Chemistry1. Functional Targets2. The Chemistry of Reactive GroupsPART IIBioconjugate Reagents3. Zero-Length Cross-Linkers4. Homobifunctional Cross-linkers5. Heterobifunctional Cross-linkers6. Trifunctional Cross-linkers7. Dendrimers and Dendrons8. Cleavable Reagent Systems9. Fluorescent Probes10. Bifunctional Chelating Agents and Radioimmunoconjugates11. Biotinylation Reagents12. Iodination Reagents13. Silane Coupling Agents14. Microparticles and Nanoparticles15. Buckyballs, Fullerenes, and Carbon Nanotubes16. Mass Tags and Isotope Tags17. Chemoselective Ligation; Bioorthogonal Reagents18. Discrete PEG CompoundsPART IIIBioconjugate Applications19. Preparation of Hapten - Carrier Immunogen Conjugates20. Antibody Modification and Conjugation21. Immunotoxin Conjugation Techniques22. Preparation of Liposome Conjugates and Derivatives23. Avidin - Biotin Systems24. Preparation of Colloidal-Gold-Labeled Proteins25. Modification with Synthetic Polymers26. Enzyme Modification and Conjugation27. Nucleic Acid and Oligonucleotide Modification and Conjugation28. Bioconjugation for the Study of Protein Interactions