Basic Methods in Molecular Biology

Edited by

  • L.G. Davis
  • M.D. Dibner
  • J.F. Battey

This innovative sourcebook contains the most recent laboratory techniques in molecular biology. Designed especially for easy access on the bench, this spiral-bound manual presents more than 80 methods in a form that facilitates quick lab set-up and replication. Each technique features an introductory section explaining its use, an estimation of the time required, a listing of the necessary equipment, a complete reagents section, step-by-step instructions and illustrations, and useful notes. The methods have been fully tested in the authors' laboratories and represent improvements and optimizations of previously published techniques.In addition to providing new methods for well-known protocols, Methods in Molecular Biology demonstrates these valuable new techniques:. Cloning into eukaryotic cells . cDNA library formation in lambda gt10 and lambda gt11 . cloning into M13 vectors . sequencing by dideoxy chain termination . in situ hybridization . transgenic mouse analysisFeaturing outstanding organization and a wealth of new material, this handy manual is an exceptional reference in the laboratory.
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Book information

  • Published: September 1986
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-01082-7

Table of Contents

The basics of molecular biology. The tools of the molecular biologist. General preparation, procedures and considerations for use of manual. Cloning vectors and bacterial cells. Preparation of DNA from eukaryotic cells. Probing nucleic acids with labeled synthetic probes. Probing nucleic acids with plasmid-derived probes. Plasmid DNA preparation. DNA restriction fragment preparation. Purification of DNA. Preparation and analysis of RNA from eukaryotic cells. Preparation of DNA from bacteriophage clones. Cloning DNA from the eukaryotic genome. cDNA cloning into gt10 and gt11. Subcloning into plasmids. Cloning and sequencing. Further characterization of cloned DNA. Transformation of mammalian cells in culture. Protein methods. General methods. Specialized methods. Appendix I: Stock solutions. Appendix II: Enzymes. Appendix III: Suppliers.