Basic Methods in Molecular BiologyEdited by
- L.G. Davis
- M.D. Dibner
- J.F. Battey
Published: September 1986
- The basics of molecular biology. The tools of the molecular biologist. General preparation, procedures and considerations for use of manual. Cloning vectors and bacterial cells. Preparation of DNA from eukaryotic cells. Probing nucleic acids with labeled synthetic probes. Probing nucleic acids with plasmid-derived probes. Plasmid DNA preparation. DNA restriction fragment preparation. Purification of DNA. Preparation and analysis of RNA from eukaryotic cells. Preparation of DNA from bacteriophage clones. Cloning DNA from the eukaryotic genome. cDNA cloning into gt10 and gt11. Subcloning into plasmids. Cloning and sequencing. Further characterization of cloned DNA. Transformation of mammalian cells in culture. Protein methods. General methods. Specialized methods. Appendix I: Stock solutions. Appendix II: Enzymes. Appendix III: Suppliers.