Applications of HPLC in Biochemistry


  • A. Fallon
  • R.F.G. Booth
  • L.D. Bell

This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.
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Book information

  • Published: July 1987
  • Imprint: ELSEVIER
  • ISBN: 978-0-444-80862-2

Table of Contents

1. The Origins and Development of Liquid Chromatography. 2. The Theory of HPLC. 3. Instrumentation. 4. High Performance Ion-Exchange Chromatography. 5. High Performance Size Exclusion Chromatography. 6. High Performance Normal Phase Chromatography. 7. High Performance Reversed Phase chromatography. 8. High Performance Reversed Phase Ion-pair Chromatography. 9. High Performance Affinity Chromatography. 10. Practical Aspects. 11. Applications of HPLC. 11.1. Nucleosides and Nucleotides. 11.2. Proteins, Peptides and Amino Acids. 11.3. Lipids. 11.4. Carbohydrates. 11.5. Prostaglandins, Leukotrienes and Hydroxyeicosatetraenoic Acids. 11.6. Steroids. 11.7. Biogenic Amines. 11.8. Vitamins. 11.9. Antibiotics. Appendix. Subject index