This volume of Methods in Enzymology and its companion Volume 226 present spectroscopic and physical methods for the determination of metal ion environments in metalloproteins and metalloenzymes.


Biochemists, Molecular and Cell Biologists, Enzymologists, Physical Chemists, Biophysicists, Analytical Chemists, Immunologists

Table of Contents

A.V. Xavier and D.L. Turner, Two-Dimensional NMR of Paramagnetic Metalloproteins. J.E. Coleman, 113Cd NMR Applied to Metalloproteins. C.F.G.C. Geraldes, Lanthanide Shift Reagents. D.E. Mota de Freitas, Alkali Metal NMR. S. Foren, C. Johansson, and S. Linse, Calcium NMR. H. Thomann and M. Bernardo, Pulsed Electron Nuclear Multiple Resonance Spectroscopic Methods for Metalloprotein and Metalloenzymes. P. Aisen and C. Bender, Continuous Wave Endor Spectroscopy. N.D. Chasteen, Vanadyl (IV) ENDOR/ESEEM Spin Probes. G.W. Canters, C.W. Hilbers, M. van de Kamp, and S.S. Wijmenga, Multidimensional NMR Methods to Probe Metal Environments in Proteins. A.J. Hoff, Optically Detected Magnetic Resonance of Triplet States in Proteins. J.R. Pilbrow and G.R. Hanson, Electron Paramagnetic Resonance. R. Cammack and C.E. Cooper, EPR Spectroscopy of Iron Complexes and Iron-Containing Proteins. B.J. Hales, Intrinsic and Extrinsic Paramagnets as Probes of Metal Clusters. M.T. Stankovich, K.E. Paulsen, and A.M. Orville, EPR-Spectroelectrochemical Titration C. Batzlaff, A.X. Trautwein, and H. Winkler, Magnetic Susceptiblity. E.P. Day, Multifield Saturation Magnetization of Metalloproteins. E. Munck, K.K. Surerus, and M.P. Hendrich, Combining Missbauer Spectroscopy with Integer Spin EPR. F.A. Armstrong, J.N. Butt, and A. Sucheta, Voltammetric Studies of Redox-Active Centers in Metalloproteins Adsorbed on Electrodes. H.A.O. Hill and N.I. Hunt, Direct and Indirect Electrochemical Investigations of Metalloenzymes. A.G. Sykes and G.A. Salmon, Pulse Radiolysis.


No. of pages:
© 1993
Academic Press
Print ISBN:
Electronic ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY