Description

Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human diseases and infections. This volume and its companion Volume 288 on Chemokine Receptors provide comprehensive experimental protocols used in this field of research.

Key Features

@introbul:Key Features @bul:* C-X-C Chemokines * C-C and C Chemokines * Other Methods

Readership

Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.

Table of Contents

C-X-C Chemokines: R. Horuk, D. Reilly, and D. Yansura, Expression, Purification, and Characterization of Escherichia coli Derived Recombinant Human Melanoma Growth Stimulating Activity. A. Wuyts, P. Proost,G. Froyen, A. Haelens, A. Billiau, G. Opdenakker, and J. Van Damme, Purification and Identification of Human and Mouse Granulocyte Chemotactic Protein-2 Isoforms. P.E. Dawson, Synthesis of Chemokines by Native Chemical Ligation. W.J. Fairbrother and H.B. Lowman, Molecular Approaches to Structure-Function Analysis of Interleukin-8. J. Hesselgesser and R. Horuk, Alanine Scan Mutagenesis of Chemokines. D. Baly, U. Gibson, D. Allison, and L. DeForge, Biological Assays for C-X-C Chemokines. K. Rajarathnam, C.M. Kay, I. Clark-Lewis, and B.D. Sykes, Characterization of Quaternary Structure of Interleukin-8 and Functional Implications. C-C and C Chemokines: J. Van Damme, S. Struyf, A. Wuyts, G. Opdenakker, P. Proost, P. Allavena, S. Sozzani, and A. Mantovani, Isolation of Human Monocyte Chemotactic Proteins and Study of their Producer and Responder Cells by Immunotests and Bioassays. A.-M. Buckle, S. Craig, and L.G. Czaplewski, Assays for Macrophage Inflammatory Proteins. P.J. Nelson, J.M. Pattison, and A.M. Krensky, Gene Expression of RANTES. A.M. Krensky and P.J. Nelson, Expression of Chemokine RANTES and Production of Monoclonal Antibodies. R.C. Newton and K. Vaddi, Biological Responses to C-C Chemokines. D.N. Cook, Gene Targeting Strategies to Study Chemokine Function in Vivo. J.A. Hedrick and A. Zlotnik, Lymphotactin: A New Class of Chemokine. J.M. Schrider, Isolation and Purific

Details

No. of pages:
421
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080883991
Print ISBN:
9780121821883

About the serial-volume-editor

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY