Electron Microscopy of Model Systems

Preface/Acknowledgements

Thomas Müller-Reichert

1. Electron microscopy of viruses

Michael Laue

2. Bacterial TEM: New insights from cryo-microscopy

Martin Pilhofer, Mark S. Ladinsky, Alasdair W. McDowall, and Grant Jensen*

3. Analysis of the ultrastructure of archaea by electron microscopy

Reinhard Rachel* , Carolin Meyer, Andreas Klingl, Sonja Gürster, Nadine Wasserburger, Ulf Küper, Annett Bellack, Simone Schopf, Reinhard Wirth, Harald Huber, and Gerhard Wanner

4. Chlamydomonas: Cryopreparation methods for the 3-D analysis of cellular organelles

Eileen T. O’Toole

5. Ultrastructure of the asexual blood stages of Plasmodium falciparum

Eric Hanssen* , Kenneth N Goldie, and Leann Tilley

6. Electron tomography and immunolabeling of Tetrahymena thermophila basal bodies

Thomas H. Giddings* Jr., Janet B. Meehl, Chad G. Pearson, and Mark Winey

7. Electron microscopy of Paramecium

Klaus Hausmann* and Richard D. Allen

8. Ultrastructural investigation methods for Trypanosoma brucei

Johanna L. Höög* , Eva Gluenz, Sue Vaughan, and Keith Gull

9. Dictyostelium discoideum: A model system for ultrastructural analyses of cell motility and development

Michael P. Koonce* and Ralpf Gräf

10. Towards sub-second correlative live-cell light and electron microscopy of Saccharomyces cerevisiae

Christopher Buser

11. Fission yeast: A cellular model well suited for electron microscopic investigations

Hélio D. Roque and Claude Antony*

12. High-pressure freezing and electron microscopy of Arabidopsis

Byung-Ho Kang

13. Standard and cryo-preparation of Hydra for transmission electron microscopy

Thomas W. Holstein* , Michael W. Hess, and Willi Salvenmoser

14. Electron microscopy of flatworms: Standard and cryopreparation methods

Willi Salvenmoser* , Bernhard Egger, Johannes G. Achatz, Peter Ladurner, and Michael W. Hess*

15. Three-dimensional reconstruction methods for C. elegans ultrastructure

Thomas Müller-Reichert* , Joel Mancuso, Ben Lich, and Kent McDonald*

16. Insects as model systems in cell biology

Thomas A. Keil* and R. Alexander Steinbrecht

17. Electron microscopy of amphibian model systems (Xenopus laevis and Ambystoma mexicanum)

Thomas Kurth* , Jürgen Berger, Michaela Wilsch-Bräuninger, Robert Cerny, Heinz Schwarz, Jan Löfberg, Thomas Piendl, and Hans Epperlein

18. Modern approaches for ultrastructural analysis of the zebrafish embryo

Nicole L. Schieber, Susan J. Nixon, Richard I. Webb* , and Robert G. Parton*

19. Transmission electron microscopy of cartilage and bone

Douglas R. Keene* , and Sara F. Tufa

20. Electron microscopy of the mouse central nervous system

Wiebke Möbius* , Frédérique Varoqueaux, Benjamin H. Cooper, Cordelia Imig, Torben Ruhwedel, Aiman S. Saab, and Walter Kaufmann

21. Rapidly excised and cryofixed rat tissue

Dimitri Vanhecke, Werner Graber, and Daniel Studer*

22. The actin cytoskeleton in whole mounts and sections

Guenter P. Resch* , Edit Urban, and Sonja Jacob

23. Three-dimensional cryo-electron microscopy on intermediate filaments: A critical comparison to other structural methods

Robert Kirmse, Cédric Bouchet-Marquis, Cynthia Page, and Andreas Hoenger*

24. Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells

Julien Guizetti, Jana Mäntler, Thomas Müller-Reichert* , and Daniel W. Gerlich*

25. Viral infection of cells in culture: Approaches for electron microscopy

Paul Walther* , Li Wang, Sandra Ließem, and Giada Frascaroli

26. Intracellular membrane traffic at high resolution

Jan R. T. van Weering, Edward Brown, Thomas H. Sharp, Judith Mantell, Peter J. Cullen, and Paul Verkade*

27. 3D versus 2D cell culture: Implications for electron microscopy

Michael W. Hess* , Kristian Pfaller, Hannes L. Ebner, Beate Beer, Daniel Hekl, and Thomas Seppi

28. ‘Tips and tricks’ for high-pressure freezing of model systems

Kent McDonald* , Heinz Schwarz, Thomas Müller-Reichert, Rick Webb, Christopher Buser, and Mary Morphew