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| DNA REPAIR, PART A, 408
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 To order this title, and for more information, click here
Judith Campbell, California Institute of Technology, Pasadena, California, U.S.A.
Paul Modrich, Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, USA
Description
This volume provides detailed coverage of modern methods for molecular analysis of enzymes and enzyme systems that function in the maintenance
of genome integrity. Coverage areas include base excision repair, nucleotide excision repair, translesion DNA polymerases, mismatch
repair, genetic recombination, and double strand break repair.
Audience
Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists
Contents
Table of Contents
Chapter 1
Assays for determining lesion bypass efficiency and mutagenicity of site-specific DNA lesions in vivo
Chapter 2
Oxidative DNA glycolases: Recipes from Cloning to Characterization
Chapter 3
Purification and Characterization of NEIL1 and
NEIL2, Members of a distinct Family of Mammalian DNA Glycosylases for repair of oxidized bases
Chapter 4
Analysis of Base Excision DNA
Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-links
Chapter 5
Isolation and analyses of
MutY and MutY Homologs (MYH)
Chapter 6
Use of yeast for detection of endogenous abasic legions, their source and their repair
Chapter
7
Activities and Mechanism of Pol Beta
Chapter 8
Direct removal of aklylation damage from DNA by AlkB and related DNA dioxygenases
Chapter
9
Purification and Characterization of DNA Photolyases
Chapter 10
Genetic and in vitro assays of DNA deamination
Chapter 11
The XPC
complex and UV-DDB: Functional assays for the damage recognition factors involved in global genome repair
Chapter 12
Purification and
Characterization of E. coli and Human Nucleotide Excision Repair
Chapter 13
Assays for transcription elongation by RNA polymerase II
using oligo (dC)-tailed
template with single DNA damage
Chapter 14In vivo assays for transcription-coupled repair
Chapter 15
TFIIh enzymatic activities in transcription and nucleotide excision repair
Chapter 16
An assay for studying ubiquitylation of RNA polymerase
II and other proteins in crude yeast extracts
Chapter 17
Analysis of the excision step in human DNA mismatch repair
Chapter 18
Characterisation
of the ?mismatch repairosome? and its role in the processing of modified nucleosides in vitro
Chapter 19
Analysis of DNA Mismatch Repair
in Cellular Response to DNA Damage
Chapter 20
Characterization of E. coli Translesion Synthesis Polymerases and Their Accessory Factors
Chapter 21
Measuring the Fidelity of Translesion DNA Synthesis
Chapter 22
DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification
and Mouse Models for Studying Their Function
Chapter 23
Purification and Characterization of Escherichia coli DNA Polymerase V
Chapter
24
Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization
Chapter 25
Localisation
of Y-family polymerases and the DNA polymerase switch in mammalian cells
Chapter 26
Repair of DNA Double Strand Breaks – in vivo Biochemistry
Chapter 27
Assays for nonhomologous end-joining in extracts
Chapter 28
Purification and assays of Saccharomyces cerevisiae homologous
recombination proteins
Chapter 29
Analysis of DNA recombination and repair proteins in living cells by photobleaching microscopy
Chapter
30
Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases
Chapter 31In Vitro Nonhomologous End
Joining Assay
Chapter 32
The RAG and HMGB1 proteins: purification and biochemical analysis of recombination signal complexes
Chapter
33
Purification and Biochemical Characterization of ATM and MRN
| Bibliographic details |
Hardbound, 624 pages, publication date: APR-2006
ISBN-13: 978-0-12-182813-4
ISBN-10: 0-12-182813-1
Imprint: ACADEMIC PRESS
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| Price and Ordering |
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USD 175
GBP 88
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Last update: 27 Sep 2008
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