Protein Expression and Purification is an international journal designed to provide biochemists, molecular biologists, and other
investigators with a forum for presenting significant advances in protein isolation. The journal publishes original articles on novel
or improved isolations of specific proteins from conventional and genetically engineered sources.
Protein Expression and Purification's
emphasis is on the application of expression and purification approaches to proteins. Findings should be novel and of general interest
to scientists involved in the protein sciences. Articles should focus on new methods which are widely applicable in the field or detailed
application of a method to an important protein. Articles must discuss the significance and/or
application of these findings and should
include a detailed description of the approaches utilized with significant characterization of a purified protein. Simple purification
strategies using common affinity purification techniques without general significance are unacceptable for publication.
In submitting
a manuscript, authors should provide a brief description of the significance to the field and suggest potential reviewers where appropriate.
Review articles describing and evaluating important advances in the expression and purification of proteins will be featured. Such reviews
are generally invited; interested authors should contact the Editor-in-Chief. Topics for original articles include but are not limited
to:
The preparation of natural and recombinant proteins:• Achievement of a high level of purity • Expression of active
recombinant proteins • Production of mutant proteins • High-throughput purification• Improved or novel fractionation
Techniques for protein expression: • Control of stability, solubility, and activity • Gene constructions designed to
facilitate purification • Vectors and hosts • High-throughput expression
Protein Expression and Purification's
emphasis is on the application of expression and purification approaches to proteins. Findings should be novel and of general interest
to scientists involved in the protein sciences. Articles should focus on new methods that are widely applicable in the field or detailed
application of a method to an important protein. Articles must discuss the significance and/or application of these findings and should
include a detailed description of the approaches used with significant characterization of a purified protein. Simple purification strategies
using common affinity techniques without general significance are unacceptable for publication.
In submitting a manuscript, authors
should provide a brief description of the significance to the field and suggest potential reviewers where appropriate.
Submission
of Manuscripts
It is a condition of publication that all manuscripts must be written in clear and grammatical English and be
submitted to the Protein Expression and Purification Web site at http://ees.elsevier.com/prep. Minimal exceptions
will be allowed. If you are unable to provide an electronic version of your paper, please contact the Editorial Office prior to submission
(e-mail: pep@elsevier.com; telephone; (619) 699-6845; fax: (619) 699-6211).
Include an abstract of 250 words or less;
do not include references in the abstract.
Include a table summarizing purification; see table instructions for specific format.
Use the reference style shown below; note that titles are included. Unpublished papers can be cited as references only if they
are in press.
Optional: Indicate members of the Editorial Board or other independent established investigators that have special
expertise to serve as reviewers of the manuscript.
There are no submission fees or page charges except for color figures
as noted below.
Manuscripts are accepted for review with the understanding that the same work has neither
been published nor is under consideration elsewhere, and that its submission for publication has been approved by all of the authors
and by the institution where the work was carried out; further, that any person cited as a source of personal communications has approved
such citation. Written authorization may be required at the editors' discretion. Articles and any other material published in Protein
Expression and Purification represent the opinions of the authors and should not be construed to reflect the opinions of the Editor(s)
or the Publisher.
Redundant or duplicate publication in another peer-reviewed primary research journal of the same paper (or substantially
similar paper) reporting the same results arising from the same research work does not serve the research or library community well,
and any such publication will be subject to review by the editor and to the retraction and removal policies of the journal.
Upon
acceptance of an article, authors will be asked to transfer copyright (for more information on copyright, see http://www.elsevier.com/copyright).
Acceptance of the agreement will ensure the widest possible dissemination of information. A letter will be sent to the corresponding
author confirming receipt of the manuscript together with a 'Journal Publishing Agreement' form or a link to the online version of this
agreement.
If material from other copyrighted works is included, the author(s) must obtain written permission from the copyright
owners and credit the source(s) in the article. Elsevier has preprinted forms for use by authors in these cases: contact Elsevier Global
Rights Department, P.O. Box 800, Oxford OX5 1DX, UK; phone: (+44) 1865 843830, fax: (+44) 1865 853333, e-mail: permissions@elsevier.com.
Preparation of Manuscript
Prepare manuscripts double-spaced throughout, including abstract, references, acknowledgments,
tables, and figure legends. Number pages consecutively. Page 1 should contain the article title, authors' names (preferably
including one full forename for each author), and complete affiliations. The first page should also include the name, mailing address,
e-mail address, and telephone and fax numbers of the author to whom correspondence and proofs should be sent. At the bottom of page 1
place any footnotes to the title. Page 2 of the manuscript should contain an abstract summarizing the accomplishments of the
study and their significance in a single paragraph of 250 words or less.
Text. The suggested organization of an article is
abstract, introductory statement, materials and methods, results, discussion, acknowledgments, and references. Some of these sections
may be combined if the presentation is thereby made clearer or more effective. In all cases an abstract is required.
Protein isolation
and expression procedures should be described in sufficient detail to allow the procedures to be used without extensive reference to
previously published studies. Well-established procedures such as methods of protein determination and general techniques of vector construction
should be cited only by literature reference, but the procedures for determining enzyme activity or for specifically quantitating nonenzymatic
proteins (e.g., immunoreactivity or densitometric quantitation of protein gels) should be described in sufficient detail to allow the
methods to be applied without reference to previous descriptions. For assay methods that have been reported previously, the description
should be as brief as is consistent with direct application of the method; the original report of the method should be cited. For novel
assay procedures, the experimental description should be complete and include evidence of specificity, sensitivity, and linearity of
response with time and amount of protein.
Vector construction should be diagrammed adequately showing relevant marker genes, regulatory
elements, insert and vector sizes, and key restriction enzyme sites. If indicated restriction enzyme sites are not unique in the construct,
this fact must be stated. Information regarding the construction of novel vectors or recombinant molecules should be provided in sufficient
detail to allow the work to be evaluated and applied. Where appropriate, DNA sequences should be reported. Previously unpublished gene
or cDNA sequences must be submitted to a public data base (e.g., GenBank/ EMBL Data Bank) prior to publication; accession numbers will
be included in the published paper as a footnote.
GenBank/DNA sequence linking. Authors wishing to enable other scientists
to use the accession numbers cited in their papers via links to these sources should type this information in the following manner:
For each and every accession number cited in an article, authors should type the accession number in bold, underlined
text. Letters in the accession number should always be capitalized (see example below). This combination of letters and format
will enable the typesetter to recognize the relevant texts as accession numbers and add the required link to GenBank's sequences.
Example: GenBank accession nos. AI631510, AI631511, AI632198, and BF223228),
a B-cell tumor from a chronic lymphatic leukemia (GenBank accession no. BE675048), and a T-cell lymphoma (GenBank accession
no. AA361117).
Authors are encouraged to check accession numbers used very carefully. An error in a letter or number
can result in a dead link. In the final version of the printed article, the accession number text will not appear bold
or underlined. In the final version of the electronic copy, the accession number text will be linked to the appropriate source
in the NCBI databases, enabling readers to go directly to that source from the article.
For articles reporting the isolation of
specific enzymes, the Results section should include a table listing each step of the procedure, and for that step the total protein,
total enzyme units, and the calculated specific activity, percentage yield, and approximate purity. For proteins without catalytic
activity, an analogous table should be included indicating for each step the total protein, the amount of specific protein of interest,
and the calculated fold enrichment and percentage yield. Figures showing chromatographic elution profiles are appropriate if the separation
being illustrated is complex and cannot adequately be described in the text.
Authors should indicate which aspects of the procedure
have been optimized or are particularly critical to the success of the procedure.
Articles describing the isolation of specific
proteins should report sufficient catalytic or physical properties of the purified protein to establish its identity. This information
(Mr, subunits, pI, pH optimum, etc.) may best be presented in tabular form. Where isoforms are known or
expected, the specific isoform purified should be identified. In general, only procedures leading to highly purified proteins will be
considered for publication. Evidence of purity should therefore be provided and may include protein gels, immunological studies, or,
for enzymes, comparison of the final specific activity with the specific activity obtained in previous studies.
Names of chemical
or organic substances should follow the recommendations of the IUPAC-IUBMB Joint Combined Commission on Biochemical Nomenclature (JCBN).
Authors should draw attention to any particular chemical or biological hazards that may be involved in carrying out the experiments
described. Any relevant safety precautions should be described; if an accepted code of practice has been followed, a reference to the
relevant standards should be given. Since it is evident that the value of a purification or expression method is entirely dependent on
the availability of all materials, sources of critical reagents and instruments must be clearly identified.
The journal accepts reports
of studies involving recombinant DNA molecules, constructed in vitro and subsequently inserted into cells, with the understanding that
the authors have adhered to appropriate NIH guidelines and/or other pertinent regulations. Procedures requiring special facilities or
precautions should be identified and described in detail.
References. Only articles that have been published or are in press
should be included in the references. Unpublished results or personal communications should be cited as such within the text. Arrange
the reference list in the order cited in the text and type double-spaced throughout. Full titles of the cited papers must be
included. Cite references by Arabic numerals, on line, in brackets, for example, [4]. The names of journals should be abbreviated according
to the Chemical Abstracts Service Source Index.
[1] R. Jayalakshmi, K. Sumathy, H. Balaram, Purification and characterization
of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli, Protein Express. Purif.
25 (2002) 66–72. [2] R. Hesketh, The Oncogene FactsBook, Academic Press, San Diego, 1995. [3] G.R. Mettam, L.B. Adams,
How to prepare an electronic version of your article, in: B.S. Jones, R.Z. Smith (Eds.) Introduction to the Electronic Age, E-Publishing
Inc., New York, 1999, pp. 281–304.
Figures. Number figures with Arabic numerals in order of appearance in the text.
Type all legends consecutively on a separate sheet. Please indicate figure number in file name and on figure. Please visit our Web site
at http://www.elsevier.com/artworkinstructions for detailed instructions on preparing electronic artwork.
Photographs
must be kept to a minimum. Magnification should be indicated by a scale where possible. Simple histograms should be avoided; a table
or a paragraph in the text is preferred.
Illustrations in color in the printed issue can be accepted only if the authors defray
the cost. However, if together with your accepted article, you submit usable color figures, then Elsevier will ensure, at no additional
charge, that these figures will appear in color on the Web (e.g., ScienceDirect and other sites) regardless of whether these illustrations
are reproduced in color in the printed version. For color reproduction in print, you will receive information regarding the costs from
Elsevier after receipt of your accepted article. Please note: Because of technical complications that can arise in converting color figures
to "gray scale" (for the printed version should you not opt for color in print), please submit in addition usable black-and-white files
corresponding to all the color illustrations.
Tables should be typed on separate pages and numbered consecutively with Arabic
numerals in order of mention in the text. Each table should have a short explanatory title.
Preparation of Supplementary Material
Elsevier accepts electronic supplementary material to support and enhance your scientific research. Supplementary files offer additional
possibilities for publishing supporting applications, movies, animation sequences, high-resolution images, background datasets, sound
clips, and more. Supplementary files supplied will be published online alongside the electronic version of your article in Elsevier Web
products, including ScienceDirect (http://www.sciencedirect.com). To ensure that your submitted material is directly usable,
please provide the data in one of our recommended file formats. Authors should submit the material in electronic format together with
the article and supply a concise and descriptive caption for each file. Please note, however, that supplementary material will not appear
in the printed journal. For in-depth information about submitting electronic artwork visit http://www.elsevier.com/artworkinstructions.
Proofs
PDF proofs will be e-mailed to the corresponding author. To avoid delay in publication, only necessary changes
should be made, and corrections should be returned promptly.
