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| HETEROTRIMERIC G PROTEINS, 237
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Ravi Iyengar, Mount Sinai School of Medicine, New York, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Included in series
Methods in Enzymology,
Description
This volume of Methods in Enzymology and its companion Volume 238 include molecular, biological, and biochemical methods
for the study of cell surface heterotrimeric G proteins (Volume 237) and their effectors (Volume 238). Methods unique to signal transducing
G proteins and general techniques applied to the study of G protein systems are covered.
Audience
Biochemists, molecular biologists, neurophysiologists, neurochemists, cell biologists, pharmacologists, and neuroendocrinologists.
Contents
Ga Subunits:
T. Wieland and K.H. Jakobs, Measurement of Receptor-Stimulated Guanosine 5'-O-((-Thio)triphosphate
Binding by G Proteins.P. Gierschik, T. Bouillon, and K.H. Jakobs, Receptor-Stimulated Hydrolysis of Guanosine 5'-Triphosphate
in Membrane Preparations.E.M. Ross and T. Higashijima, Regulation of G-Protein Activation by Mastoparans and Other Cationic
Peptides.D.J. Carty and R. Iyengar, Guanosine 5'-O-((-Thio)triphosphate Binding Assay for Solubilized G Proteins.
J. Moss, R.S. Haun, S.-C. Tsai, C.F. Welsh, F.-J.S. Lee, S.R. Price, and M. Vaughan, Activation of Cholera Toxin by ADP-Ribosylation
Factors: 20-kDa Guanine Nucleotide-Binding Proteins.D.J. Carty, Pertussis Toxin-Catalyzed ADP-Ribosylation of G Proteins.R.R.
Vaillancourt, N. Dhanasekaran, and A.E. Ruoho, Synthesis and Use of Radioactive Photoactivatable NAD+ Derivatives as Probes for
G-Protein Structure.M.M. Rasenick, M. Talluri, and W.J. Dunn, III, Photoaffinity Guanosine 5'-Triphosphate Analogs as a Tool
for the Study of GTP-Binding Proteins.L. Birnbaumer, D. Grenet, F. Ribeiro-Neto, and J. Codina, Preparation of Activated (
Subunits of Gs and Gis: From Erythrocyte to Activated Subunit.T. Katada, K. Kontani, A. Inanobe, I. Kobayashi, Y. Ohoka, H. Nishina,
and K. Takahashi, Purification and Separation of Closely Related Members of Pertussis Toxin-Substrate G Proteins.J. Bigay and
M. Chabre, Purification of Transducin.E. Lee, M.E. Linder, and A.G. Gilman, Expression of G-Protein a Subunits in Escherichia
coli.I.-H. Pang, A.V. Smrcka, and P.C. Sternweis, Synthesis and Applications of Affinity Matrix Containing Immobilized
(( Subunits of G Proteins.J.L. Blank and J.H. Exton, Purification of Activated and Heterotrimeric Forms of Gq Proteins.G.L.
Waldo, J.L. Boyer, and T.K. Harden, Purification of Phospholipase C-Activating G Protein, G11, from Turkey Erythrocytes.J.R.
Hepler, T. Kozasa, and A.G. Gilman, Purification of Recombinant Gqa, G11a, and G16a from Sf9 Cells.S.G. Graber, R.A. Figler,
and J.C. Garrison, Expression and Purification of G-Protein a Subunits Using Baculovirus Expression System.E.J. Neer, B.M.
Denker, T.C. Thomas, and C.J. Schmidt, Analysis of G-Protein a and (Subunits by in Vitro Translation.Y. Audigier,
Assays for Studying Functional Properties of in Vitro Translated Gsa Subunit.S.M. Mumby and M.E. Linder, Myristoylation
of G- Protein a Subunits.G. Milligan, Specificity and Functional Applications of Antipeptide Antisera Which Identify G-Protein
a Subunits.K.-L. Laugwitz, K. Spicher, G. Schultz, and S. Offermanns, Identification of Receptor-Activated G Proteins: Selective
Immunoprecipitation of Photolabeled G-Protein a Subunits.J. Lyons, Identification of Mutant Forms of G-Protein a Subunits in
Human Neoplasia by Polymerase Chain Reaction-Based Techniques.P.V. Gejman and L.S. Weinstein, Detection of Mutations and Polymorphisms
of the Gsa Subunit Gene by Denaturing Gradient Gel Electrophoresis.S. Winitz, M. Russell, and G.L. Johnson, Construction of
Mutant and Chimeric G-Protein a Subunits.T.M. Wilkie, A.M. Aragay, A.J. Watson, and M.I. Simon, Design of Degenerate Oligonucleotide
Primers for Cloning of G-Protein a Subunits.C. Kleuss, G. Schultz, and B. Wittig, Microinjection of Antisense Oligonucleotides
to Assess G-Protein Subunit Function.R.M. Mortensen and J.G. Seidman, Inactivation of G-protein Genes: Double Knockout in Cell
Lines.U. Rudolph, A. Bradley, and L. Birnbaumer, Targeted Inactivation of the Gi2( Gene with Replacement and Insertion Vectors:
Analysis in a 96-Well Plate Format.B.E. Snaar-Jagalska and P.J.M. Van Haastert, G-Protein Assays in Dictyostelium.
R.A. Cerione, Fluorescence Assays for G-Protein Interactions.H.E. Hamm and H.M. Rarick, Specific Peptide Probes for
G-Protein Interactions with Receptors.F. Quan and M. Forte, Vaccinia Virus Systems for Expression of G( Genes in S49 Cells.
Gbg Subunits:
J. Bigay and M. Chabre, Purification of Tbg Subunit of Transducin.J. Chen, D.J. Carty, and
R. Iyengar, Adenylyl Cyclase Assay for bg Subunits of G Proteins.J. Dingus, M.D. Wilcox, R. Kohnken, and J.D. Hildebrandt,
Synthesis and Use of Biotinylated bg Complexes Prepared from Bovine Brain G Proteins.C. Gallagher and N. Gautam, Design of
Oligonucleotide Probes for Molecular Cloning of b and g Subunits.A.N. Pronin and N. Gautam, Characterization of Antibodies
for Various G-Protein b and g Subunits.J.D. Robishaw and E.A. Balcueva, Preparation, Characterization, and Use of Antibodies
with Specificity for G-Protein g Subunits.B.K.-K. Fung, J.S. Anant, W.-C. Lin, O.C. Ong, and H.K. Yamane, Isoprenylation of
g Subunits and G-Protein Effectors.
Author Index.
Subject Index.
| Bibliographic details |
Hardbound, 561 pages, publication date: JUN-1994
ISBN-13: 978-0-12-182138-8
ISBN-10: 0-12-182138-2
Imprint: ACADEMIC PRESS
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