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CILIA: STRUCTURE AND MOTILITY, 91
Cilia: Structure and Motility, 91
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Stephen King
Gregory Pazour

Included in series
Methods in Cell Biology,

Contents


1. RNAi approaches to axonemal motor function in trypanosomes (Hill)
2. Homologous recombination to knock out dynein genes in Tetrahymena (Gaertig)
3. RNAi in Chlamydomonas (Cerutti)
4. Planarians as a model system for analysis of ciliary assembly/motility (Rompolas)
5. Role of nucleotides in dynein function (Shingyoji)
6. Protein modification to probe dynein interactions + purification and identification of crosslinked dynein products (Sakato)
7. Analysis of redox-sensitive dynein components (Wakabayashi)
8. Purification of dyneins from Chlamydomonas and analysis of dynein-dynein linkers (Kamiya/King)
9. Kinase/phosphatase-mediated control of dynein function (Sale)
10. Central pair MT complex and associated kinesins (Mitchell)
11. Calcium regulation of axonemal function In vitro motility assays (Smith)
12. Identification and analysis of dynein regulatory complex components (Porter)
13. Isolation and analysis of radial spoke proteins (Yang)
14. Purification of dyneins from other model organisms: Ciona, sea urchin, fish (Inaba)
15. Rescue of mutant phenotypes by protein electroporation (Kamiya Lab)
16. Tubulin/Dynein interactions (Raff)
17. Measurement of beat frequency and swimming velocity Cell model reactivation in vitro assays of dynein function (Kamiya)
18. Live imaging of ependymal cilia (Lechtreck/Witman)
19. Immunogold labeling of axonemal components in situ + flat embedding (Geimer)
20. CryoEM approaches to axonemal organization (Nicastro)
21. CryoEM of dynein-microtubule complexes (Oda/Kikkawa)
22. Bioinformatic approaches to dynein HC classification (Yagi)
23. Bioinformatics of non-motor dynein components (Asai)
24. Biophysical approaches to dynein mechanism (Sutoh)
25. Chlamydomonas flagellar beat analysis (Foster)
26. Biophysical measurements of motor function (step size/force production) SAXS of axonemes (Toba/Oiwa)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 



Bibliographic details
Hardbound, 344 pages, publication date: NOV-2009
ISBN-13: 978-0-12-374973-4
Imprint: ACADEMIC PRESS

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Last update: 20 Nov 2009
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