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| HUMAN STEM CELL MANUAL
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A Laboratory Guide
To order this title, and for more information, click here
Edited By
Jeanne Loring, The Burnham Institute, La Jolla, California, U.S.A.
Robin Wesselschmidt
Philip Schwartz
Audience
Scientists engaged in any aspect of stem cell research, as well as undergraduate and graduate students enrolled in laboratory courses.
Contents
Foreword by James Battey
Foreword by Jeannie Fontana
Preface
Part I: Basic methods: Maintenance of stem cell cultures
1.Human Embryonic
Stem Cell Culture
2.Feeder layers and feeder-free culture
3.Mouse Embryonic Fibroblast Feeder Cells
4.Cryopreservation of hESC
Part
II: Basic methods: Characterization of stem cells
5.Classical Cytogenetics: Karyotyping
6.Spectral Karyotyping and Fluorescent in situ
Hybridization
7.Using SNP Genotyping to Identify Cell Lines, Determine Genomic Abnormalities and Monitor DNA Methylation Patterns
8.FACS/Cell
sorting
9.Fluorescence Immunocytochemical Analysis of Stem Cells
10.Characterization of Stem Cells Using RT-PCR
11.Gene Expression Profiling
of Stem Cells by Microarray
12.Teratomas produced from Human Embryonic Stem Cells Xenografted into Immunodeficient Mice
13.Generation
of HESC-Derived Teratomas
Part III: Differentiation of stem cells
14.?Embryoid Body? Formation and Neuroepithelia Differentiation
15.Motor
Neuron and Dopamine Neuron Differentiation
16.Oligodendrocyte Differentiation from hESC
17.Cardiac
18.Hematopoietic
Part IV: Genetic
manipulation of stem cells
19.Genetic Manipulation of hESC: Lentivirus Vectors
20.Methods developed for mouse ES cells
Part V: Advanced
methods
21.Derivation of human ES cells
22.Neural Stem Cell Culture
23.Transplantation of stem cells
24.In vitro fertilization
25.Functional
characterization-neural cells
Part VI: Practical issues in establishing a human ES cell laboratory
26.Setting Up a Facility for hESC
Research
Part VII: Essays on stem cell biology
27.Intellectual Property: Owning the Stem Cell
28.Ethical Concerns for Stem Cell Research
29.Guidelines for hESC Research Oversight (ESCRO) Committees
| Bibliographic details |
Spiral Bound, 488 pages, publication date: JUN-2007
ISBN-13: 978-0-12-370465-8
ISBN-10: 0-12-370465-0
Imprint: ACADEMIC PRESS
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USD 84.95
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Last update: 25 Nov 2009
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