Description Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking
of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly
after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed
since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches.
It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new
yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental
protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.
Audience
Microbiologists and Yeast researchers (yeast molecular biologists, cell biologists) C.elegans/mouse/other eukaryote system researchers possibly seeking to utilise yeast as a simple model system
Contents Preface
Introduction to functional analysis in yeast
Yeast genetics and strain construction
Yeast transformation
A guided tour to PCR-based
genomic manipulations of S. cerevisiae (PCR-targeting)
Studying essential genes:
generating and using promoter fusions and
conditional alleles
Yeast hybrid approaches
Array-based yeast two-hybrid screening for protein-protein interactions
Reporter genes and
their uses in studying yeast gene expression
Transcript analysis: a microarray approach
GFP-based microscopic approaches for whole chromosome
analysis in yeasts
Immunological methods
Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer
between CFP and YFP
Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins
Yeast protein microarrays
Smart genetic screens
High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae
Chemical genomic tools for understanding gene function and drug action
RNA gene analysis
Analysis of gene function of mitochondria
Yeast
prions and their analysis in vivo
Metabolic control in the eukaryotic cell, a Systems Biology perspective
Phylogenetic footprintingSaccharomyces
cerevisiae as a tool for human gene function discovery
Bioinformatic prediction of yeast gene function
Yeast genetic strain and
plasmid collections
Yeast gene analysis: the future
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