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By David Engelke, University of Michigan, Ann Arbor, U.S.A. John Rossi, City of Hope's Beckman Research Institute, Duarte, California, U.S.A.
Description The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications
in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and
reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields
of life sciences.
RNA Interference will cover RNAi in non-vertebrates (plants, C. elegans, drosophila, and S. pombe),
and Mammalian systems (human and non-human cells). This volume discusses extensive methodology related to delivery methods high throughput
strategies and prospects as a human therapy agent.
Contents Transgene-induced RNA Interference as a Tool for Plant Functional Genomics
Constructs and Methods for hairpin RNA-mediated Gene Silencing
in Plants
RNA interference in Caenorhabditis elegans
High-throughput RNAi screens in Drosophila tissue culture cells
Mechanistic insights
aid computational siRNA design
Novel methods for expressing RNAi in human cells
Delivery of Small Interfering RNA to Mammalian Cells
in Culture Using Cationic Lipid/Polymer-Based Transfection Reagents
The subcellular distribution of siRNA: directed delivery through
RNA polymerase III expression cassettes and localization using in situ hybridization
Viral delivery of recombinant shRNAs
Downregulation
of cellular genes by PCR cassettes expressing short interfering RNAs
Using short hairpin RNA expression vectors to study mammalian neural
development
Analysis of T-cell development using siRNA to knockdown protein expression
Viral delivery of recombinant siRNA expression:
lentivirus
Paradigms for Conditional Expression of RNAi Molecules for use Against Viral Targets
High throughput RNAi strategies for target
discovery and validation using synthetic siRNAs: functional genomics investigations of biological pathways
RNA interference in vivo:
towards synthetic siRNA based therapeutics
Labeling and characterization of small RNAs associated with the RNAi effector complex, RITS
RNAi spreading in C. elegans
Human Dicer: Purification, properties, and interaction with PAZ PIWI domain proteins
Delivery of siRNA and
siRNA Expression Constructions to Adult Mammals Using Hydrodynamic Intravascular Injection
Analysis of siRNA function in RNAi using Drosophila
Embryo extracts and Schneider cells
Using RNA polymerase II to transcribe artificial microRNAs
Adeno-associated virus vectors for short
hairpin RNA expression
Simple, Robust Strategies for Generating ddRNAi Constructs
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