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| PROTEOME ANALYSIS
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Interpreting the Genome
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Edited By
David Speicher, The Wistar Institute, Philadelphia, PA 19104.
Description
This book explores the current status of proteomics, an exciting new discipline, which is less than 10 years old. This new field has
rapidly grown into a major commercial and research enterprise with great prospects for dramatically advancing our knowledge of basic
biological and disease processes. The contributors to this book are an international panel of proteomics experts, who review and discuss
the current status of specific technologies and approaches.
Proteomics represents an exciting new way to pursue biological and biomedical
science at an unprecedented pace. Proteomics takes a broad, comprehensive, systematic approach to understanding biology that is generally
unbiased and not dependent upon existing knowledge. The major components of proteomics from basic discovery using a range of alternative
analytical methods to discovery validation and use for clinical applications are discussed. State-of-the-art protein profiling methods
include high resolution two-dimensional gels, two-dimensional differential in-gel electrophoresis, LC-MS and LC-MS/MS using accurate
mass tags, and protein identifications of proteins from gels using mass spectrometry methods are discussed in depth. Other chapters
describe comprehensive characterization of proteomes using electrophoretic prefractionation and analyses of sub-proteomes based on specific
posttranslational modifications including the phospho-proteome, the glyco-proteome, and nitrated proteins. These conventional proteome
analysis chapters are complemented by discussion of emerging technologies and approaches such as affinity based biosensor proteomics
as well as the use of protein microarrays, microfluidics and nanotechnology. Strategies for improving throughput by automation are also
discussed. Additional chapters address the application of current proteome techniques to clinical problems and the availability of protein
expression library resources for proteome studies.
Audience
Graduate and undergraduate students studying physical or analytical chemistry techniques like mass spectrometry, analytical separation
and electrophoresis; students in medicinal chemistry biology, biochemistry, cell biology, genomics and other biological sciences. Scientists
working in Biotech and Pharmaceutical companies, university researchers in Biology, Life-Science and Chemistry departments, and any scientists
working in institutions conducting biomedical research.
Contents
Overview of proteome analysis.
Protein profile comparisons of microorganisms, cells and tissues using 2-D gels.
Protein profiling
using 2-Dimensional Gel Electrophoresis (2D-DIGE) with multiple fluorescent tags.
Electrophoretic prefractionation for comprehensive
analysis of proteomes.
Modification specific proteomics applied to protein glycosylation and nitration.
Phosphoproteomics: Mass
spectrometry based techniques for systematic phosphoprotein analysis.
Protein identification by in-gel digestion and mass spectrometry.
The use of accurate mass tags based upon high-throughput Fourier transform ion cyclotron resonance mass spectrometry for global proteomic
characterization.
Clinical applications of proteomics.
Affinity-based biosensors, microarrays and proteomics.
Protein expression
library resources for proteome studies.
Automation of proteome analysis.
Micro- and nanotechnology for proteomics.
| Bibliographic details |
Hardbound, 394 pages, publication date: MAR-2004
ISBN-13: 978-0-444-51024-2
ISBN-10: 0-444-51024-9
Imprint: ELSEVIER
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| Price and Ordering |
Price:
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GBP 138.99
EUR 163.95
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Last update: 7 Sep 2009
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