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| DEVELOPMENT OF SEA URCHINS, ASCIDIANS, AND OTHER INVERTEBRATE DEUTEROSTOMES: EXPERIMENTAL APPROACHES, 74
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Charles Ettensohn, Carnegie Mellon University, Pittsburgh, PA, U.S.A.
Gary Wessel, Brown University, Providence, RI, U.S.A.
Gregory Wray, Duke University, Durham, NC, U.S.A.
Description
This book provides a practical guide to experimental methods for studying the development invertebrate deuterostomes as animal model systems.
The chapters provide detailed experimental protocols that cover a broad range of topics in modern experimental methods. Topics covered
range from rearing embryos to the care of adult animals, while also presenting the basic experimental methods including light and electron
microscopy, used to study gene expression, transgenics, reverse genetics, and genomic approaches.
Audience
Developmental biologists and cell biologists.
Contents
Overview: Echinoderms and other invertebrate deuterostomes as experimental material for the study of cell and developmental biology; I.
Procurement, Maintenance and Culture of Oocytes, Embryos, Larvae, and Adults; Care and maintenance of adult echinoderms (species, breeding
seasons, suppliers, care and handling, aquarium maintenance,large-scale aquaculture); Echinoderm eggs and embryos: Procurement and culture
(small and large scale); Culture of echinoderm larvae through metamorphosis; Isolation and maturation of echinoderm oocytes; Procurement
and culture of ascidian embryos; Culture of adult ascidians and ascidian genetics; Hemichordate embryos - procurement, culture, and basic
methods; Cephalochordate embryos - procurement, culture, and basic methods. II. Embryological
Approaches; Microinjection into eggs,
blastomeres, and the blastocoel; Blastomere isolation and transplantation; Isolation and culture of micromeres and PMCs; Transplantation
and ablation of cells at later stages; Cell adhesion assays. III. Cell Biological Approaches; Immunostaining; Light microscopy of embryos;
Electron microscopy of embryos; Calcium imaging; Imaging components of cells with fluorescent probes; Isolating extracellular matrices;
Isolating organelles; Measuring ion fluxes in gametes. IV. Molecular Biological Approaches; Isolating DNA, RNA, polysomes, and protein;
m-RNA detection by in situ hybridization and RT-PCR; Using reporters to study cis-regulatory elements; Nuclear extracts, footprinting
mobility shift assays, affinity purification; Expression of exogenous mRNAs; Disruption of gene function using antisense morpholinos
and function-blocking antibodies; Generation and use of transgenic ascidian embryos. V. Genomics; Genomic resources for echinoderms:
Sequence databases, arrayed libraries and filters; Genomic resources for ascidians: Sequence/expression databases and genome projects;
Gene network analysis. VI. Echinoderm Eggs and Embryos in the Teaching Lab; Simple and reliable laboratory exercises. VII. Appendices.
| Bibliographic details |
Hardbound, 912 pages, publication date: OCT-2004
ISBN-13: 978-0-12-480278-0
ISBN-10: 0-12-480278-8
Imprint: ACADEMIC PRESS
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| Price and Ordering |
Price:
USD 150
EUR 106.95
GBP 90.99
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Last update: 3 Oct 2009
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